Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region

• Published in: Molecular plant pathology Vol. 12; no. 6; pp. 535 - 547
• Main Authors: VERA RUIZ, ELSA MARÍA, SORIANO, JOSÉ MIGUEL, ROMERO, CARLOS, ZHEBENTYAYEVA, TETYANA, TEROL, JAVIER, ZURIAGA, ELENA, LLÁCER, GERARDO, ABBOTT, ALBERT GLENN, BADENES, MARÍA LUISA
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.2011; Blackwell
• Subjects: Bacterial artificial chromosomes; Biological and medical sciences; Chromosome Mapping; Chromosome Segregation - genetics; Chromosomes, Artificial, Bacterial - genetics; Contig Mapping; Crops; Disease resistance; Disease Resistance - genetics; DNA probes; Fingerprinting; Fruits; Fundamental and applied biological sciences. Psychology; Gene mapping; Genes, Plant - genetics; Genetic Association Studies; Genetic Loci - genetics; Genetic Markers; Genome, Plant - genetics; Genomes; Genotype; Haplotypes; Homology; Limiting factors; Microsatellite Repeats - genetics; Nucleotide sequence; Oligonucleotides; Original; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - virology; plum pox; Plum pox virus; Plum Pox Virus - immunology; Prunus; Prunus - genetics; Prunus - immunology; Prunus - virology; Prunus armeniaca; Simple sequence repeats; Synteny; Synteny - genetics; Plant pathogen; Rosaceae; Peach; Apricot; Virus; Resistance; Plum pox virus; Dicotyledones; Angiospermae; Prunus armeniaca; Spermatophyta; Fruit tree; Potyvirus; Genome; Locus; Potyviridae; Comparative study
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/j.1364-3703.2010.00691.x

SUMMARY Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high‐density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of ‘Lito’ and ‘Goldrich’ PPV‐resistant cultivars, respectively. Using these maps, and excluding genotype–phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’ and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30–40 cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by high‐information content fingerprinting (HICF). In addition, a total of 300 BAC‐derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold_1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified.