超高效液相色谱-串联质谱法同时检测干果中16种真菌毒素
建立了同时检测10种常见干果中16种真菌毒素的超高效液相色谱-串联质谱方法。将干果可食部分加水匀浆,以10 mmol/L柠檬酸-乙腈溶液为提取剂,C_（18）为净化剂,使用ACQUITY UPLC BEH C_（18）色谱柱分离样品,在优化的洗脱梯度和时间条件下,16种真菌毒素在8 min内实现良好分离,检出限为0.02~1.00μg/L;在线性范围（1~200μg/L）内线性相关系数R〉0.9981,3个添加水平的平均回收率为70.48%~118.85%,相对标准偏差（RSD）为0.3%~11.9%。本方法经济、快速、简单、高效,能够满足不同干果基质中多种真菌毒素同时检测要求。...
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| Published in | 分析化学 Vol. 45; no. 10; pp. 1556 - 1563 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国农业科学院果树研究所/农业部果品质量安全风险评估实验室(兴城),兴城,125100
2017
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 建立了同时检测10种常见干果中16种真菌毒素的超高效液相色谱-串联质谱方法。将干果可食部分加水匀浆,以10 mmol/L柠檬酸-乙腈溶液为提取剂,C_（18）为净化剂,使用ACQUITY UPLC BEH C_（18）色谱柱分离样品,在优化的洗脱梯度和时间条件下,16种真菌毒素在8 min内实现良好分离,检出限为0.02~1.00μg/L;在线性范围（1~200μg/L）内线性相关系数R〉0.9981,3个添加水平的平均回收率为70.48%~118.85%,相对标准偏差（RSD）为0.3%~11.9%。本方法经济、快速、简单、高效,能够满足不同干果基质中多种真菌毒素同时检测要求。 |
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| Bibliography: | Mycotoxins; Ultra high performance liquid chromatography-tandem mass spectrometry; Dried fruits A method for simultaneous detection of multi-mycotoxins in ten kinds of common dried fruits was been exploited by using ultra performance liquid chromatography-tandem mass spectrometry.The edible part of dried fruits was ground up with certain quantity of water.The mash was extracted by 10 mmol/L citric acidacetonitrile,purified by C_（18） column,and filtered using 0.22-μm organic filtration.All the sixteen mycotoxins were well separated in 8 min on ACQUITY UPLC BEH C_（18） column with acetonitrile as phase A and 10 mmol/L ammonium acetate solution containing 0.1% formic acid as mobile phase B.Result showed that the limits of detection（LODs） for the sixteen mycotoxins were 0.01-1.00 μg/L,with linearity range of 1-200 μg/L and correlation coefficients above 0.9981.The average recoveries of the sixteen mycotoxins in ten matrices were 70.48%-118.85%,and the relative standard deviation（RSD,n=6） was 0.3%-11.9%.This economi |
| ISSN: | 0253-3820 |
| DOI: | 10.11895/j.issn.0253-3820.170186 |