Transcriptome analyses of the Giardia lamblia life cycle
We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the...
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Published in | Molecular and biochemical parasitology Vol. 174; no. 1; pp. 62 - 65 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.11.2010
Amsterdam: Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of G. lamblia. |
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Bibliography: | http://dx.doi.org/10.1016/j.molbiopara.2010.05.010 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Current Address: Section of Microbiology, University of California at Davis, Davis, CA 95616, USA Current Address: Sanford-Burnham Medical Research, La Jolla, CA 92037, USA Current Address: Andrew McArthur Consulting, 11 Roanoke Road, Hamilton, Ontario L8S 3P6, Canada Current Address: Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA Current Address: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA Current Address: European Centre for Disease Prevention and Control, SE-171 83 Stockholm, Sweden |
ISSN: | 0166-6851 1872-9428 1872-9428 |
DOI: | 10.1016/j.molbiopara.2010.05.010 |