How Diverse Is the Genus Wolbachia? Multiple-Gene Sequencing Reveals a Putatively New Wolbachia Supergroup Recovered from Spider Mites (Acari: Tetranychidae)

• Published in: Applied and Environmental Microbiology Vol. 75; no. 4; pp. 1036 - 1043
• Main Authors: Ros, Vera I.D, Fleming, Vicki M, Feil, Edward J, Breeuwer, Johannes A.J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.2009; American Society for Microbiology (ASM)
• Subjects: Acari; Adaptations; Animals; Arthropods; Bacteria; Bacterial Proteins - genetics; Bacteriology; bacterium wolbachia; Biological and medical sciences; Cluster Analysis; cytoplasmic incompatibility; dna amplification; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Evolution, Molecular; f-supergroup; filarial nematodes; Fundamental and applied biological sciences. Psychology; Genes; Genes, rRNA; genome sequence; Invertebrate Microbiology; Microbiology; Molecular Sequence Data; Nematodes; phylogenetic analysis; Phylogeny; pipientis; Polymorphism, Genetic; Proteins; Recombination, Genetic; reproductive incompatibility; Ribonucleic acid; RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Tetranychidae - microbiology; Wolbachia - classification; Wolbachia - genetics; Wolbachia - isolation & purification; Rickettsiales; Wolbachieae; Arachnida; Acari; Gene; Wolbachia; Arthropoda; Bacteria; Invertebrata; Sequencing
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.01109-08

At least 20% of all arthropods and some nematode species are infected with intracellular bacteria of the genus WOLBACHIA: This highly diverse genus has been subdivided into eight "supergroups" (A to H) on the basis of nucleotide sequence data. Here, we report the discovery of a new Wolbachia supergroup recovered from the spider mite species Bryobia species V (Acari: Tetranychidae), based on the sequences of three protein-coding genes (ftsZ, gltA, and groEL) and the 16S rRNA gene. Other tetranychid mites possess supergroup B Wolbachia strains. The discovery of another Wolbachia supergroup expands the known diversity of Wolbachia and emphasizes the high variability of the genus. Our data also clarify the existing supergroup structure and highlight the use of multiple gene sequences for robust phylogenetic analysis. In addition to previous reports of recombination between the arthropod-infecting supergroups A and B, we provide evidence for recombination between the nematode-infecting supergroups C and D. Robust delineation of supergroups is essential for understanding the origin and spread of this common reproductive parasite and for unraveling mechanisms of host adaptation and manipulation across a wide range of hosts.