CD8+ Tregs ameliorate inflammatory reactions in a murine model of allergic rhinitis

• Published in: Allergy, asthma, and clinical immunology Vol. 17; no. 1; pp. 1 - 74
• Main Authors: Lin, Lin, Dai, Fei, Wei, Jinjin, Chen, Zheng
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 22.07.2021; BioMed Central; BMC
• Subjects: Allergic rhinitis; Analysis; Asthma; CD8+CD25+ Foxp3+ regulatory T cells; Cell culture; Chronic illnesses; Cytokines; Flow cytometry; Interleukins; Investigations; Laboratory animals; Lymphocytes; Mouse; Nasal mucosa; Rhinitis; Software; Statistical analysis; T cell receptors; T cells; Transforming growth factors; California; United States > US; China; Germany
• ISSN: 1710-1492; 1710-1484; 1710-1492
• DOI: 10.1186/s13223-021-00577-8

Background CD8.sup.+CD25.sup.+fork-head box transcription factor (Foxp3).sup.+ regulatory T cells (CD8.sup.+ Tregs) play a role in immune tolerance. However, the role of these cells in allergic rhinitis (AR) has not been elucidated. The study aimed to evaluate influences of CD8.sup.+ Tregs on inflammatory conditions in a murine model of AR. Methods A murine model of AR was established. CD8.sup.+ Tregs were isolated from mice nasal mucosa and cultured in vitro. We examined interleukin (IL)-10 and transforming growth factor (TGF)-[beta] in cell cultures. Then, we administered CD8.sup.+ Tregs into mice nasal mucosal cultures, and examined eosinophil cation protein (ECP), IL-4, IL-5 and IL-13 in these cultures. Finally, we adoptively transferred CD8.sup.+ Tregs into mice models, and evaluated percentages of CD8.sup.+ Tregs, numbers of sneezing and nasal rubbing, and counts of eosinophils and contents of ECP, IL-4, IL-5, IL-13, IL-10 and TGF-[beta] in nasal lavage fluid (NLF) in mice. Results The percentage of CD8.sup.+ Tregs from AR mice was reduced. IL-10 and TGF-[beta] were increased in cell cultures from AR mice. ECP, IL-4, IL-5 and IL-13 were decreased after the AR mice CD8.sup.+ Tregs administration in mucosal cultures. However, their contents were not changed after normal CD8.sup.+ Tregs treatment. Additionally, the adoptive transfer of AR CD8.sup.+ Tregs enhanced the percentage of CD8.sup.+ Tregs and levels of IL-10 and TGF-[beta] in NLF, reduced numbers of sneezing and nasal rubbing, and counts of eosinophils and concentrations of ECP, IL-4, IL-5 and IL-13 in NLF. However, normal CD8.sup.+ Tregs could not change above parameters. Conclusion These findings show that CD8.sup.+ Tregs may inhibit inflammatory responses in the AR condition. Keywords: CD8.sup.+CD25.sup.+ Foxp3.sup.+ regulatory T cells, Allergic rhinitis, Mouse, Model, Nasal mucosa