Development of a new parallelized, optical biosensor platform for label-free detection of autoimmunity-related antibodies

• Published in: Analytical and bioanalytical chemistry Vol. 406; no. 14; pp. 3305 - 3314
• Main Authors: Bleher, Oliver, Schindler, Aline, Yin, Meng-Xin, Holmes, Andrew B., Luppa, Peter B., Gauglitz, Günter, Proll, Günther
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2014; Springer; Springer Nature B.V
• Subjects: Analysis; Analytical Chemistry; Antibodies; Antibodies, Anticardiolipin - immunology; Antigen-Antibody Reactions; Antigens; Antiphospholipid Syndrome - immunology; autoantibodies; Autoantibodies - immunology; autoimmune diseases; Autoimmunity; Autoimmunity - immunology; beta 2-Glycoprotein I - chemistry; Biochemistry; Biosensing Techniques; Biosensors; blood serum; Cardiolipins - chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Control equipment; Enzyme-Linked Immunosorbent Assay; Equipment Design; Food Science; Glass; Glycoproteins; Health aspects; Heterogeneity; Humans; image analysis; Laboratory Medicine; Microscopy, Interference; Monitoring/Environmental Analysis; Multiplex Platforms in Diagnostics and Bioanalytics; Patients; Proteins; Prothrombin - chemistry; reflectometry; Research Paper; Sensitivity and Specificity; Serums; Silanes - chemistry; Spectrophotometry; spectroscopy; Spots; Viral antibodies; Germany; Reflectometric interference spectroscopy; Label-free detection; Optical sensors; Microarray; β2-Glycoprotein I; Antiphospholipid syndrome
• ISSN: 1618-2642; 1618-2650; 1618-2650
• DOI: 10.1007/s00216-013-7504-y

Autoimmune diseases are characterized by the presence of autoantibodies in serum of affected patients. The heterogeneity of autoimmune relevant antigens creates a variety of different antibodies, which requires a simultaneous detection mode. For this reason, we developed a tool for parallelized, label-free, optical detection that accomplishes the characterization of multiple antigen–antibody interactions within a single measurement on a timescale of minutes. Using 11-aminoundecyltrimethoxysilane, we were able to immobilize proteinogenic antigens as well as an amino-functionalized cardiolipin on a glass surface. Assay conditions were optimized for serum measurements with a single spot antigen chip on a single spot 1-λ detection system. Minimized background signal allows a differentiation between patients and healthy controls with a good sensitivity and specificity. Applying polarized imaging reflectometric interference spectroscopy, we evaluated samples from three APS patients and three control subjects for this proof-of-principle and already obtained good results for β2-glycoprotein I and cardiolipin.