Washingtonia filifera seed extracts inhibit the islet amyloid polypeptide fibrils formations and α-amylase and α-glucosidase activity

• Published in: Journal of enzyme inhibition and medicinal chemistry Vol. 36; no. 1; pp. 517 - 524
• Main Authors: Floris, Sonia, Fais, Antonella, Medda, Rosaria, Pintus, Francesca, Piras, Alessandra, Kumar, Amit, Kuś, Piotr Marek, Westermark, Gunilla Torstensdotter, Era, Benedetta
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2021; Taylor & Francis Group
• Subjects: alpha-amylase; alpha-Amylases - antagonists & inhibitors; alpha-Amylases - metabolism; alpha-glucosidase; alpha-Glucosidases - metabolism; Arecaceae - chemistry; Dose-Response Relationship, Drug; enzyme inhibition; Glycoside Hydrolase Inhibitors - chemistry; Glycoside Hydrolase Inhibitors - isolation & purification; Glycoside Hydrolase Inhibitors - pharmacology; Humans; islet amyloid polypeptide; Islet Amyloid Polypeptide - antagonists & inhibitors; Islet Amyloid Polypeptide - metabolism; Molecular Docking Simulation; Molecular Structure; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Research Paper; Seeds - chemistry; Structure-Activity Relationship; Washingtonia filifera; α-amylase; α-glucosidase; islet amyloid polypeptide; α-glucosidase; α-amylase; enzyme inhibition; Washingtonia filifera
• ISSN: 1475-6366; 1475-6374; 1475-6374
• DOI: 10.1080/14756366.2021.1874945

Washingtonia filifera seeds have revealed to possess antioxidant properties, butyrylcholinesterase and xanthine oxidase inhibition activities. The literature has indicated a relationship between Alzheimer's disease (AD) and type-2 diabetes (T2D). Keeping this in mind, we have now evaluated the inhibitory properties of W. filifera seed extracts on α-amylase, α-glucosidase enzyme activity and the Islet Amyloid Polypeptide (IAPP) fibrils formation. Three extracts from seeds of W. filifera were evaluated for their enzyme inhibitory effect and IC 50 values were calculated for all the extracts. The inhibition mode was investigated by Lineweaver-Burk plot analysis and the inhibition of IAPP aggregate formation was monitored. W. filifera methanol seed extract appears as the most potent inhibitor of α-amylase, α-glucosidase, and for the IAPP fibril formation. Current findings indicate new potential of this extract that could be used for the identification or development of novel potential agents for T2D and AD.