Chitosan-tethered poly(acrylonitrile- co-maleic acid) hollow fiber membrane for lipase immobilization

• Published in: Biomaterials Vol. 26; no. 32; pp. 6394 - 6403
• Main Authors: Ye, Peng, Xu, Zhi-Kang, Che, Ai-Fu, Wu, Jian, Seta, Patrick
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.11.2005; Elsevier
• Subjects: Acrylic Resins - analysis; Acrylic Resins - chemistry; Adsorption; Biomimetic Materials - analysis; Biomimetic Materials - chemistry; Candida rugosa; Chemical Sciences; Chitosan; Chitosan - analysis; Chitosan - chemistry; Coated Materials, Biocompatible - chemistry; Dual-layer biomimetic membrane; Enzyme Activation; Enzyme immobilization; Enzyme Stability; Enzymes, Immobilized - chemistry; Hydrogen-Ion Concentration; Lipase; Lipase - analysis; Lipase - chemistry; Materials Testing; Membranes, Artificial; Poly(acrylonitrile- co-maleic acid) membrane; Porosity; Temperature; Dual-layer biomimetic membrane; Enzyme immobilization; Chitosan; Lipase; Poly(acrylonitrile- co-maleic acid) membrane
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2005.04.019

A protocol was used to prepare a dual-layer biomimetic membrane as support for enzyme immobilization by tethering chitosan on the surface of poly(acrylonitrile- co-maleic acid) (PANCMA) ultrafiltration hollow fiber membrane in the presence of 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC)/ N-hydroxylsuccin-imide (NHS). The chemical change of the chitosan-modified PANCMA membrane surface was confirmed with Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Lipase from Candida rugosa was immobilized on this dual-layer biomimetic membrane using glutaraldehyde (GA), and on the nascent PANCMA membrane using EDC/NHS as coupling agent. The properties of the immobilized enzymes were assayed and compared with those of the free one. It was found that both the activity retention of the immobilized lipase and the amount of bound protein on the dual-layer biomimetic membrane (44.5% and 66.5 mg/m 2) were higher than those on the nascent PANCMA membrane (33.9% and 53.7 mg/m 2). The kinetic parameters of the free and immobilized lipases, K m and V max, were also assayed. The K m values were similar for the immobilized lipases, while the V max value of the immobilized lipase on the dual-layer biomimetic membrane was higher than that on the nascent PANCMA membrane. Results indicated that the pH and thermal stabilities of lipase increased upon immobilization. The residual activity of the immobilized lipase after 10 uses was 53% on the dual-layer biomimetic membrane and 62% on the nascent PANCMA membrane.