vLUME: 3D virtual reality for single-molecule localization microscopy

• Published in: Nature methods Vol. 17; no. 11; pp. 1097 - 1099
• Main Authors: Spark, Alexander, Kitching, Alexandre, Esteban-Ferrer, Daniel, Handa, Anoushka, Carr, Alexander R., Needham, Lisa-Maria, Ponjavic, Aleks, Santos, Ana Mafalda, McColl, James, Leterrier, Christophe, Davis, Simon J., Henriques, Ricardo, Lee, Steven F.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.11.2020; Nature Publishing Group
• Subjects: 631/114/794; 631/57/2265; Algorithms; Animals; Bioinformatics; Biological Microscopy; Biological properties; Biological samples; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Brief Communication; Caulobacter crescentus - chemistry; Cell Line; Cell Membrane - chemistry; Chlorocebus aethiops; Clathrin - chemistry; Computer applications; COS Cells; Equipment and supplies; Humans; Image Processing, Computer-Assisted - methods; Jurkat Cells; Life Sciences; Localization; Methods; Microscope and microscopy; Microscopy; Microtubules - chemistry; Molecular biology; Nuclear Pore - chemistry; Proteomics; Segmentation; Single Molecule Imaging - methods; Software; Three dimensional analysis; Virtual Reality; Visualization; Visualization (Computers); United Kingdom
• ISSN: 1548-7091; 1548-7105
• DOI: 10.1038/s41592-020-0962-1

v LUME is a virtual reality software package designed to render large three-dimensional single-molecule localization microscopy datasets. v LUME features include visualization, segmentation, bespoke analysis of complex local geometries and exporting features. v LUME can perform complex analysis on real three-dimensional biological samples that would otherwise be impossible by using regular flat-screen visualization programs. v LUME is a complete virtual reality environment for visualizing, analyzing and interacting with three-dimensional single-molecule localization microscopy data.