Molecular analysis and antimicrobial susceptibility of enterotoxigenic Escherichia coli from diarrheal patients

• Published in: Diagnostic microbiology and infectious disease Vol. 81; no. 2; pp. 126 - 131
• Main Authors: Pan, Haijian, Zhang, Jianmin, Kuang, Dai, Yang, Xiaowei, Ju, Wenting, Huang, Zheng, Guo, Jiayin, Li, Yong, Zhang, Pingping, Shi, Weimin, Jin, Huiming, Shi, Xianming, Xu, Xuebin, Meng, Jianghong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2015
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents - pharmacology; Antimicrobial susceptibility; Child; Child, Preschool; China - epidemiology; Diarrhea - epidemiology; Diarrhea - microbiology; Enterotoxigenic Escherichia coli - classification; Enterotoxigenic Escherichia coli - drug effects; Enterotoxigenic Escherichia coli - genetics; Enterotoxigenic Escherichia coli - isolation & purification; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; ETEC; Female; Genotype; Humans; Infant; Infant, Newborn; Infectious Disease; Internal Medicine; Male; Middle Aged; MLST; Molecular Epidemiology; Molecular Typing; PFGE; Virulence Factors - genetics; Virulence gene; Young Adult; China; PFGE; Antimicrobial susceptibility; MLST; ETEC; Virulence gene
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2014.10.008

Abstract A total of 123 enterotoxigenic Escherichia coli (ETEC) isolates from diarrheal patients from June to December 2012 in Shanghai, China, were examined to determine their genetic relatedness using multilocus sequence typing and pulsed-field gel electrophoresis (PFGE) and for the presence of virulence genes and antimicrobial susceptibility. Twenty-nine sequence types (STs) and 63 PFGE patterns were identified, and results from the 2 subtyping methods correlated well. The 12 isolates of PFGE cluster B all belonged to ST-2332 and were associated with nosocomial neonatal diarrhea. Isolates of a cluster usually had the same set of virulence factors, whereas isolates of different PFGE clusters carried diverse combinations of virulence determinants. Isolates belonging to ST-2332 and ST-182 ( n = 9) were resistant to at least 6 antimicrobials. Our findings highlighted the need of active surveillance programs for infectious diseases collecting data at both epidemiological and genetic levels that can detect high-risk lineages of pathogens in order to rapidly identify disease outbreaks.