Isolation and characterization of glucose derepressed invertase mutants from Schizosaccharomyces pombe

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 12; pp. 2475 - 2478
• Main Authors: Kig, C.(Istanbul Univ. (Turkey)), Turkel, S, Temizkan, G
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.12.2005; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Antimetabolites - pharmacology; beta-Fructofuranosidase - chemistry; beta-Fructofuranosidase - genetics; beta-Fructofuranosidase - isolation & purification; Biological and medical sciences; CONSOMMATION; CONSUMO; CONSUMPTION; Deoxyglucose - pharmacology; FRUCTOFURANOSIDASA; FRUCTOFURANOSIDASE; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Fungal - genetics; GENETIC ENGINEERING; GENIE GENETIQUE; GLUCOSA; GLUCOSE; Glucose - pharmacology; glucose consumption; glucose repression; INGENIERIA GENETICA; inv1 gene; invertase; MUTANT; MUTANTES; MUTANTS; Mutation - genetics; Mutation - physiology; Schizosaccharomyces - drug effects; Schizosaccharomyces - enzymology; Schizosaccharomyces - genetics; SCHIZOSACCHAROMYCES POMBE; Schizosaccharomyces pombe Proteins - genetics; Schizosacchoromyces pombe; Yeast; Enzyme; β-Fructofuranosidase; glucose consumption; invl gene; Glucose; Schizosacchoromyces pombe; Fungi; Characterization; Gene; invertase; Glycosidases; Schizosaccharomyces pombe; Hydrolases; Isolation; Ascomycetes; Mutation; O-Glycosidases; Thallophyta; glucose repression
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.69.2475

We have isolated 14 different Schizosaccharomyces pombe mutants that synthesize invertase enzyme constitutively. Analyses of invertase activities revealed that the degrees of resistance to glucose repression were not similar among different complementation groups. One of the complementation groups appeared to be associated with functional and/or regulatory defects in hexose transport. Another complementation group appeared to be specific for the regulation of the inv1 gene alone, implying that these mutations might be associated with different genes acting on the glucose sensing and signaling pathway. In addition, we found that the wild-type level glucose uptake is essential for the full-level repression of inv1 expression.