Efficient propagation of progressive multifocal leukoencephalopathy-type JC virus in COS-7-derived cell lines stably expressing Tat protein of human immunodeficiency virus type 1

• Published in: Microbiology and immunology Vol. 54; no. 12; p. 758
• Main Authors: Nukuzuma, Souichi, Nakamichi, Kazuo, Kameoka, Masanori, Sugiura, Shigeki, Nukuzuma, Chiyoko, Miyoshi, Isao, Takegami, Tsutomu
• Format: Journal Article
• Language: English
• Published: Australia 01.12.2010
• Subjects: Animals; Cercopithecus aethiops; COS Cells; HIV-1 - physiology; JC Virus - growth & development; tat Gene Products, Human Immunodeficiency Virus - physiology
• ISSN: 0385-5600
• DOI: 10.1111/j.1348-0421.2010.00278.x

The high incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients compared with many other immunosuppressive diseases suggests that HIV-1 infection is strictly related to the activation of JC virus (JCV) propagation. In this report, propagation of PML-type JCV in COS-7-derived cell lines stably expressing HIV-1 Tat (COS-tat cells) has been examined. In COS-tat cells, production of viral particles and replication of genomic DNA were markedly increased compared to COS-7 cells, as judged by HA and real-time PCR analyses. These results demonstrate that COS-tat cells provide a useful model system for studying HIV-1 Tat-mediated propagation of PML-type JCV.