合浦珠母贝选育家系的遗传多样性分析
该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝（Pinctada fucata）完全双列杂交家系的遗传多样性进行了分析。6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因（Ne）为1.758 7-3.586 5,观测杂合度（Ho）为0.144 4-0.488 9,期望杂合度（He）为0.432 0-0.722 2,Shannon指数（I）为0.691 9-1.507 4。9个家系都有单态位点,平均Ho为0.129 2-0.466 7,平均He为0.155 0-0.439 6,平均I为0.248 5-0.712 2。有19个位点（占35.19%）极显著地偏离H...
Saved in:
| Published in | 南方水产科学 Vol. 12; no. 5; pp. 90 - 96 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300
2016
上海海洋大学水产与生命学院,上海201306 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0780 |
| DOI | 10.3969/j.issn.2095-0780.2016.05.011 |
Cover
| Abstract | 该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝（Pinctada fucata）完全双列杂交家系的遗传多样性进行了分析。6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因（Ne）为1.758 7-3.586 5,观测杂合度（Ho）为0.144 4-0.488 9,期望杂合度（He）为0.432 0-0.722 2,Shannon指数（I）为0.691 9-1.507 4。9个家系都有单态位点,平均Ho为0.129 2-0.466 7,平均He为0.155 0-0.439 6,平均I为0.248 5-0.712 2。有19个位点（占35.19%）极显著地偏离Hardy-Weinberg平衡。各家系之间的遗传距离为0.109 0-1.137 2,遗传相似性系数为0.320 7-0.896 8。家系L4B46与L4B48的遗传距离最大,与D3D313的遗传距离最小。UPGMA法聚类分析显示,9个家系分为3支,L4B48单独成一支,B4D426、B4D427与D4B445聚成一支,其余家系聚成一支。该研究结果为合浦珠母贝家系选择育种的亲本选择与交配设计提供了科学依据。 |
|---|---|
| AbstractList | 该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝（Pinctada fucata）完全双列杂交家系的遗传多样性进行了分析。6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因（Ne）为1.758 7-3.586 5,观测杂合度（Ho）为0.144 4-0.488 9,期望杂合度（He）为0.432 0-0.722 2,Shannon指数（I）为0.691 9-1.507 4。9个家系都有单态位点,平均Ho为0.129 2-0.466 7,平均He为0.155 0-0.439 6,平均I为0.248 5-0.712 2。有19个位点（占35.19%）极显著地偏离Hardy-Weinberg平衡。各家系之间的遗传距离为0.109 0-1.137 2,遗传相似性系数为0.320 7-0.896 8。家系L4B46与L4B48的遗传距离最大,与D3D313的遗传距离最小。UPGMA法聚类分析显示,9个家系分为3支,L4B48单独成一支,B4D426、B4D427与D4B445聚成一支,其余家系聚成一支。该研究结果为合浦珠母贝家系选择育种的亲本选择与交配设计提供了科学依据。 S917.4; 该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝(Pinctada fucata)完全双列杂交家系的遗传多样性进行了分析.6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因(Ne)为1.758 7～3.586 5,观测杂合度(Ho)为0.144 4～0.488 9,期望杂合度(He)为0.432 0 ～0.722 2,Shannon指数(I)为0.691 9～1.507 4.9个家系都有单态位点,平均H0为0.129 2～0.466 7,平均He为0.155 0 ～0.439 6,平均I为0.248 5～0.712 2.有19个位点(占35.19％)极显著地偏离Hardy-Weinberg平衡.各家系之间的遗传距离为0.109 0～1.137 2,遗传相似性系数为0.320 7～0.896 8.家系L4B46与L4B48的遗传距离最大,与D3D313的遗传距离最小.UPGMA法聚类分析显示,9个家系分为3支,L4B48单独成一支,B4D426、B4D427与D4B445聚成一支,其余家系聚成一支.该研究结果为合浦珠母贝家系选择育种的亲本选择与交配设计提供了科学依据. |
| Author | 范嗣刚 王婧璇 黄桂菊 刘宝锁 郭奕惠 喻达辉 |
| AuthorAffiliation | 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300 上海海洋大学水产与生命学院,上海201306 |
| AuthorAffiliation_xml | – name: 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300;上海海洋大学水产与生命学院,上海201306 |
| Author_FL | GUO Yihui LIU Baosuo YU Dahui FAN Sigang WANG Jingxuan HUANG Guiju |
| Author_FL_xml | – sequence: 1 fullname: FAN Sigang – sequence: 2 fullname: WANG Jingxuan – sequence: 3 fullname: HUANG Guiju – sequence: 4 fullname: LIU Baosuo – sequence: 5 fullname: GUO Yihui – sequence: 6 fullname: YU Dahui |
| Author_xml | – sequence: 1 fullname: 范嗣刚 王婧璇 黄桂菊 刘宝锁 郭奕惠 喻达辉 |
| BookMark | eNo9j81Kw0AUhWdRwVr7EqLLxDszmUxmKcU_KLjpPkwnk5qiE00QcVdFpF2I3QkFpQvRnYK_C30ck7RvYaTi6hwO37mXs4AqJjYaoWUMNhWuWO3aUZoam4BgFnAPSoddG5gNGFdQ9T-fR_U0jdqAucN5Wawimg37-dt9cTXOny4nrzfT3mBy9pw9vhcvn8XofHp6_f01zu5G-fgj7z1k_Yv8driI5kK5l-r6n9ZQa2O91diymjub2421pqUYxxYDDUIyFchAYuG0lRZMMhc7HtXE8aTLuNBScKWpIAFxlWAcJHE0UZ5HBdAaWpmdPZYmlKbjd-OjxJQPfROm6nchsHJfyS3NOLUbm85hVJIHSbQvkxPf5UCpwxinPwTVZpM |
| ClassificationCodes | S917.4 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.2095-0780.2016.05.011 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-农业科学 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| DocumentTitleAlternate | Analysis of genetic diversity of nine families of Pinctada fucata |
| DocumentTitle_FL | Analysis of genetic diversity of nine families of Pinctada fucata |
| EndPage | 96 |
| ExternalDocumentID | nfsc201605011 670334557 |
| GrantInformation_xml | – fundername: 现代农业(贝类)产业技术体系建设专项资金; 国家自然科学基金面上项目; 广东省自然基金博士启动项目; 海南省自然科学基金项目; 广东省海洋渔业科技与产业发展专项 funderid: (CARS-48); (31372525); (2014A030310237); (20154180); (Z2015010,Z2015009) |
| GroupedDBID | -04 2B. 2B~ 2RA 5VS 92G 92I 92L ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CQIGP ECGQY GROUPED_DOAJ KQ8 TCJ TGD U1G U5N W95 ~WA 4A8 93N PSX UY8 |
| ID | FETCH-LOGICAL-c571-50e09a5cdada194bce95a561483e248a6579ea97ce392d26c9570a24e2c883903 |
| ISSN | 2095-0780 |
| IngestDate | Thu May 29 04:08:32 EDT 2025 Wed Feb 14 10:12:09 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Keywords | 合浦珠母贝 genetic diversity 微卫星 遗传多样性 mating design microsatellite 家系选育 Pinctada fucata 交配设计 family selection |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c571-50e09a5cdada194bce95a561483e248a6579ea97ce392d26c9570a24e2c883903 |
| Notes | Pinctada fucata; family selection; microsatellite; genetic diversity; mating design We studied the genetic diversity of breeding family of Pinctada fucata. Six microsatellite markers were chosen for genetic analysis of 360 individuals of nine families. A total of 32 alleles were detected and the number of allele at each locus was 3 - 8. The effective allele was 1. 758 7 - 3. 586 5. The observed heterozygosity（ Ho） was 0. 144 4 - 0. 488 9. The expected heterozygosity（ He）was 0. 432 0 - 0. 722 2 and the Shannon index was 0. 691 9 - 1. 507 4. Monomorphism loci were found in each family. The average Ho（ 0. 129 2 - 0. 466 7）,average He（ 0. 155 0 - 0. 439 6） and Shannon index（ 0. 248 5 - 0. 712 2） were detected in family population.Nineteen loci（ 35. 19%） were deviated from Hardy-Weinberg equilibrium test significantly. The genetic distance was 0. 109 0 -1. 137 2 and the genetic identify index was 0. 320 7 - 0. 896 8. L4B46 was the furthest with L4B48 and the nearest with D3D313 in terms of genetic distance. Nei＇s g |
| PageCount | 7 |
| ParticipantIDs | wanfang_journals_nfsc201605011 chongqing_primary_670334557 |
| PublicationCentury | 2000 |
| PublicationDate | 2016 |
| PublicationDateYYYYMMDD | 2016-01-01 |
| PublicationDate_xml | – year: 2016 text: 2016 |
| PublicationDecade | 2010 |
| PublicationTitle | 南方水产科学 |
| PublicationTitleAlternate | South China Fisheries Science |
| PublicationTitle_FL | South China Fisheries Science |
| PublicationYear | 2016 |
| Publisher | 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300 上海海洋大学水产与生命学院,上海201306 |
| Publisher_xml | – name: 上海海洋大学水产与生命学院,上海201306 – name: 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300 |
| SSID | ssib017477969 ssib051370567 ssib007891391 ssib038075195 ssib036438877 ssj0001560132 |
| Score | 2.0226207 |
| Snippet | 该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝（Pinctada fucata）完全双列杂交家系的遗传多样性进行了分析。6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因（Ne）为1.758 7-3.586 5,观测杂合度（Ho）为0.144 4-0.488... S917.4; 该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝(Pinctada fucata)完全双列杂交家系的遗传多样性进行了分析.6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因(Ne)为1.758 7～3.586 5,观测杂合度(Ho)为0.144 4～0.488... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 90 |
| SubjectTerms | 交配设计 合浦珠母贝 家系选育 微卫星 遗传多样性 |
| Title | 合浦珠母贝选育家系的遗传多样性分析 |
| URI | http://lib.cqvip.com/qk/60642B/201605/670334557.html https://d.wanfangdata.com.cn/periodical/nfsc201605011 |
| Volume | 12 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw7V07b9RAELaigBAUiKcIgShFtrywfqx3p7TvfIpAICEFKd3JZ_uS6gIhaVIFhFAoEOmQIoFSIOhA4lnAz-EuyX-gYGbW90gUIECbxlrvrr_9ZmbvPGOvZx1nCsLMa_pFWglM6lWCNCsqkFMuQvCbGd6gW4bfnt-8Fc7cCa7PqbmR4z-GVi2tLDens9UDvyv5F6tiHdqVvpL9C8v2QbECy2hfPKKF8XgoG4tECZDCGJGEIlYiCkWihamLSFJNVBemJhIj4kAAFoCWNRigGuOJ2KPLo0TEfFXsizimAkTCBNzZFaBFEoi4yoDYOaBWQpYi1lRAwEhTE3IwIdVAIuzOnD2Xl1trDIWtyBOYrSRWBB4xgqYjuDxKDQXpTQQmWxXGZ1mxl1-OBtGgC8qcCBPzxVDCgScG91gSB6UjuZC8S-ITLupnCMXCmlIrVmFArvbwkxH7ySbPYmZviC5Ri0VcO4xgXCMJmArA2sdxkLa3R1NoTqviAwD3XR6SUWkNZ5URUMvVkhtZC0h5kfkFuGGofmdDTVZNOByw1dH8hguoLxKEO6PCrcKQA2k74CFwLM1zTvMMg54uE2qKIrKkrcGZVLLdR4mhoM4IwOD_SYmHwG7lT6Xas3Pcm45yjx3jOqnLckP-eFoqZ7iGqUJCaSJpk3N1NBOOZoIv5cCp8iRlpNB297i-B-gN3enUkDtX_l_awMDuvL3f5fQhBHY5CX66D0-LhkObE9oduNr9BdBtDHCoh1SSElMc87R2aUH6jdsDb0jTCpJBJjNXB1rjYL1zH2NJ9BD7wSPtHGI3W7XnyvU1Rrd68MaBHr7xjp19liecqVKEa78TgPIOLSy25-9hNMUft7ZbaXt-KA6bPeOcLh-gTEb2bnjWGVldOOeciuaXyiRSxXnH72ysdz-93n621X33dOfji921JzsP33feft7-8HV789Hug-ffv211Xm12t75019501h93X25ccGbryWx1plJuDlTJlHYrShYSUpXlaZ66EDSzAlTKWa39wkO_I1QaihR0Vvjg5V6YgdIy9YLCy4zxQfoXndH2Yru45EwGrieVKUyuMBjHCLyZ577xWiAhbwZhyx1zxvvSN-7aHFCNED1lP1BKjzkTpT4apWdwv7HHupf_1GHcOUll-1z_ijO6vLRSXMVId7k5wRPiJ1JdUXs |
| linkProvider | Colorado Alliance of Research Libraries |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%90%88%E6%B5%A6%E7%8F%A0%E6%AF%8D%E8%B4%9D%E9%80%89%E8%82%B2%E5%AE%B6%E7%B3%BB%E7%9A%84%E9%81%97%E4%BC%A0%E5%A4%9A%E6%A0%B7%E6%80%A7%E5%88%86%E6%9E%90&rft.jtitle=%E5%8D%97%E6%96%B9%E6%B0%B4%E4%BA%A7%E7%A7%91%E5%AD%A6&rft.au=%E8%8C%83%E5%97%A3%E5%88%9A&rft.au=%E7%8E%8B%E5%A9%A7%E7%92%87&rft.au=%E9%BB%84%E6%A1%82%E8%8F%8A&rft.au=%E5%88%98%E5%AE%9D%E9%94%81&rft.date=2016&rft.pub=%E4%B8%AD%E5%9B%BD%E6%B0%B4%E4%BA%A7%E7%A7%91%E5%AD%A6%E7%A0%94%E7%A9%B6%E9%99%A2%E5%8D%97%E6%B5%B7%E6%B0%B4%E4%BA%A7%E7%A0%94%E7%A9%B6%E6%89%80%2C%E5%86%9C%E4%B8%9A%E9%83%A8%E5%8D%97%E6%B5%B7%E6%B8%94%E4%B8%9A%E8%B5%84%E6%BA%90%E5%BC%80%E5%8F%91%E4%B8%8E%E5%88%A9%E7%94%A8%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E5%8D%97%E6%B5%B7%E7%94%9F%E7%89%A9%E8%B5%84%E6%BA%90%E5%BC%80%E5%8F%91%E4%B8%8E%E5%88%A9%E7%94%A8%E5%8D%8F%E5%90%8C%E5%88%9B%E6%96%B0%E4%B8%AD%E5%BF%83%2C%E5%B9%BF%E4%B8%9C%E5%B9%BF%E5%B7%9E510300%25%E4%B8%AD%E5%9B%BD%E6%B0%B4%E4%BA%A7%E7%A7%91%E5%AD%A6%E7%A0%94%E7%A9%B6%E9%99%A2%E5%8D%97%E6%B5%B7%E6%B0%B4%E4%BA%A7%E7%A0%94%E7%A9%B6%E6%89%80%2C%E5%86%9C%E4%B8%9A%E9%83%A8%E5%8D%97%E6%B5%B7%E6%B8%94%E4%B8%9A%E8%B5%84%E6%BA%90%E5%BC%80%E5%8F%91%E4%B8%8E%E5%88%A9%E7%94%A8%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E5%8D%97%E6%B5%B7%E7%94%9F%E7%89%A9%E8%B5%84%E6%BA%90%E5%BC%80%E5%8F%91%E4%B8%8E%E5%88%A9%E7%94%A8%E5%8D%8F%E5%90%8C%E5%88%9B%E6%96%B0%E4%B8%AD%E5%BF%83%2C%E5%B9%BF%E4%B8%9C%E5%B9%BF%E5%B7%9E510300&rft.issn=2095-0780&rft.volume=12&rft.issue=5&rft.spage=90&rft.epage=96&rft_id=info:doi/10.3969%2Fj.issn.2095-0780.2016.05.011&rft.externalDocID=nfsc201605011 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F60642B%2F60642B.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fnfsc%2Fnfsc.jpg |