合浦珠母贝选育家系的遗传多样性分析

该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝（Pinctada fucata）完全双列杂交家系的遗传多样性进行了分析。6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因（Ne）为1.758 7-3.586 5,观测杂合度（Ho）为0.144 4-0.488 9,期望杂合度（He）为0.432 0-0.722 2,Shannon指数（I）为0.691 9-1.507 4。9个家系都有单态位点,平均Ho为0.129 2-0.466 7,平均He为0.155 0-0.439 6,平均I为0.248 5-0.712 2。有19个位点（占35.19%）极显著地偏离H...

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Published in南方水产科学 Vol. 12; no. 5; pp. 90 - 96
Main Author 范嗣刚 王婧璇 黄桂菊 刘宝锁 郭奕惠 喻达辉
Format Journal Article
LanguageChinese
Published 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发与利用重点实验室,南海生物资源开发与利用协同创新中心,广东广州510300 2016
上海海洋大学水产与生命学院,上海201306
Subjects
交配设计
合浦珠母贝
家系选育
微卫星
遗传多样性
合浦珠母贝
genetic diversity
微卫星
遗传多样性
mating design
microsatellite
家系选育
Pinctada fucata
交配设计
family selection
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ISSN2095-0780
DOI10.3969/j.issn.2095-0780.2016.05.011

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Summary:该研究选取具有多态性的6对微卫星引物对构建的2批合浦珠母贝（Pinctada fucata）完全双列杂交家系的遗传多样性进行了分析。6个微卫星标记在9个家系360个个体中共检测到32个等位基因,有效等位基因（Ne）为1.758 7-3.586 5,观测杂合度（Ho）为0.144 4-0.488 9,期望杂合度（He）为0.432 0-0.722 2,Shannon指数（I）为0.691 9-1.507 4。9个家系都有单态位点,平均Ho为0.129 2-0.466 7,平均He为0.155 0-0.439 6,平均I为0.248 5-0.712 2。有19个位点（占35.19%）极显著地偏离Hardy-Weinberg平衡。各家系之间的遗传距离为0.109 0-1.137 2,遗传相似性系数为0.320 7-0.896 8。家系L4B46与L4B48的遗传距离最大,与D3D313的遗传距离最小。UPGMA法聚类分析显示,9个家系分为3支,L4B48单独成一支,B4D426、B4D427与D4B445聚成一支,其余家系聚成一支。该研究结果为合浦珠母贝家系选择育种的亲本选择与交配设计提供了科学依据。
Bibliography:Pinctada fucata; family selection; microsatellite; genetic diversity; mating design
We studied the genetic diversity of breeding family of Pinctada fucata. Six microsatellite markers were chosen for genetic analysis of 360 individuals of nine families. A total of 32 alleles were detected and the number of allele at each locus was 3 - 8. The effective allele was 1. 758 7 - 3. 586 5. The observed heterozygosity（ Ho） was 0. 144 4 - 0. 488 9. The expected heterozygosity（ He）was 0. 432 0 - 0. 722 2 and the Shannon index was 0. 691 9 - 1. 507 4. Monomorphism loci were found in each family. The average Ho（ 0. 129 2 - 0. 466 7）,average He（ 0. 155 0 - 0. 439 6） and Shannon index（ 0. 248 5 - 0. 712 2） were detected in family population.Nineteen loci（ 35. 19%） were deviated from Hardy-Weinberg equilibrium test significantly. The genetic distance was 0. 109 0 -1. 137 2 and the genetic identify index was 0. 320 7 - 0. 896 8. L4B46 was the furthest with L4B48 and the nearest with D3D313 in terms of genetic distance. Nei＇s g
ISSN:2095-0780
DOI:10.3969/j.issn.2095-0780.2016.05.011