ROS Production by a Single Neutrophil Cell and Neutrophil Population upon Bacterial Stimulation

• Published in: Biomedicines Vol. 11; no. 5; p. 1361
• Main Authors: Pleskova, Svetlana N., Erofeev, Alexander S., Vaneev, Alexander N., Gorelkin, Petr V., Bobyk, Sergey Z., Kolmogorov, Vasilii S., Bezrukov, Nikolay A., Lazarenko, Ekaterina V.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.05.2023; MDPI
• Subjects: Analysis; B cells; Bacteria; Carbon; Cytochrome; Cytoplasm; E coli; electrochemical detection; Electrodes; Escherichia coli; Fibroblasts; Genotype & phenotype; Leukocytes (granulocytic); Leukocytes (neutrophilic); Methods; Morphology; neutrophil heterogeneity; Neutrophils; Population levels; Quantum dots; Reactive oxygen species; ROS production; S. aureus; United States > US; Russia; S. aureus; neutrophil heterogeneity; luminol-dependent chemiluminescence; ROS production; electrochemical detection; neutrophils; E. coli; nanoelectrodes

The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, f...