ROS Production by a Single Neutrophil Cell and Neutrophil Population upon Bacterial Stimulation

• Published in: Biomedicines Vol. 11; no. 5; p. 1361
• Main Authors: Pleskova, Svetlana N., Erofeev, Alexander S., Vaneev, Alexander N., Gorelkin, Petr V., Bobyk, Sergey Z., Kolmogorov, Vasilii S., Bezrukov, Nikolay A., Lazarenko, Ekaterina V.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.05.2023; MDPI
• Subjects: Analysis; B cells; Bacteria; Carbon; Cytochrome; Cytoplasm; E coli; electrochemical detection; Electrodes; Escherichia coli; Fibroblasts; Genotype & phenotype; Leukocytes (granulocytic); Leukocytes (neutrophilic); Methods; Morphology; neutrophil heterogeneity; Neutrophils; Population levels; Quantum dots; Reactive oxygen species; ROS production; S. aureus; United States > US; Russia; S. aureus; neutrophil heterogeneity; luminol-dependent chemiluminescence; ROS production; electrochemical detection; neutrophils; E. coli; nanoelectrodes
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines11051361

The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a “silent cell” to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of S. aureus was 5.5-fold greater than that produced under the influence of E. coli. The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with S. aureus, as compared to stimulation with E. coli, caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.