ROS Production by a Single Neutrophil Cell and Neutrophil Population upon Bacterial Stimulation

The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, f...

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Published inBiomedicines Vol. 11; no. 5; p. 1361
Main Authors Pleskova, Svetlana N., Erofeev, Alexander S., Vaneev, Alexander N., Gorelkin, Petr V., Bobyk, Sergey Z., Kolmogorov, Vasilii S., Bezrukov, Nikolay A., Lazarenko, Ekaterina V.
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 04.05.2023
MDPI
Subjects
Analysis
B cells
Bacteria
Carbon
Cytochrome
Cytoplasm
E coli
electrochemical detection
Electrodes
Escherichia coli
Fibroblasts
Genotype & phenotype
Leukocytes (granulocytic)
Leukocytes (neutrophilic)
Methods
Morphology
neutrophil heterogeneity
Neutrophils
Population levels
Quantum dots
Reactive oxygen species
ROS production
S. aureus
United States > US
Russia
S. aureus
neutrophil heterogeneity
luminol-dependent chemiluminescence
ROS production
electrochemical detection
neutrophils
E. coli
nanoelectrodes
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Abstract The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a “silent cell” to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of S. aureus was 5.5-fold greater than that produced under the influence of E. coli. The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with S. aureus, as compared to stimulation with E. coli, caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.
AbstractList The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a “silent cell” to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of S. aureus was 5.5-fold greater than that produced under the influence of E. coli. The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with S. aureus, as compared to stimulation with E. coli, caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.
The reactive oxygen species (ROS) production by a single neutrophil after stimulation with and was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a "silent cell" to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of was 5.5-fold greater than that produced under the influence of . The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with , as compared to stimulation with , caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.
The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a “silent cell” to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of S. aureus was 5.5-fold greater than that produced under the influence of E. coli . The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with S. aureus , as compared to stimulation with E. coli , caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.
The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a "silent cell" to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of S. aureus was 5.5-fold greater than that produced under the influence of E. coli. The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with S. aureus, as compared to stimulation with E. coli, caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical amperometric method with a high time resolution. This showed significant variability in the response of a single neutrophil to bacterial stimulation, from a "silent cell" to a pronounced response manifested by a series of chronoamperometric spikes. The amount of ROS produced by a single neutrophil under the influence of S. aureus was 5.5-fold greater than that produced under the influence of E. coli. The response of a neutrophil granulocyte population to bacterial stimulation was analyzed using luminol-dependent biochemiluminescence (BCL). The stimulation of neutrophils with S. aureus, as compared to stimulation with E. coli, caused a total response in terms of ROS production that was seven-fold greater in terms of the integral value of the light sum and 13-fold greater in terms of the maximum peak value. The method of ROS detection at the level of a single cell indicated the functional heterogeneity of the neutrophil population, but the specificity of the cellular response to different pathogens was the same at the cellular and population levels.
Audience Academic
Author Erofeev, Alexander S.
Gorelkin, Petr V.
Bezrukov, Nikolay A.
Bobyk, Sergey Z.
Pleskova, Svetlana N.
Vaneev, Alexander N.
Kolmogorov, Vasilii S.
Lazarenko, Ekaterina V.
AuthorAffiliation 3 Laboratory of Biophysics, National University of Science and Technology MISIS, Leninskiy Prospect, 4, 119049 Moscow, Russia
1 Laboratory of Scanning Probe Microscopy, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, Russia
4 Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, Russia
2 Department “Nanotechnology and Biotechnology”, R.E. Alekseev Technical State University of Nizhny Novgorod, 603155 Nizhny Novgorod, Russia
AuthorAffiliation_xml – name: 3 Laboratory of Biophysics, National University of Science and Technology MISIS, Leninskiy Prospect, 4, 119049 Moscow, Russia
– name: 4 Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, Russia
– name: 1 Laboratory of Scanning Probe Microscopy, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, Russia
– name: 2 Department “Nanotechnology and Biotechnology”, R.E. Alekseev Technical State University of Nizhny Novgorod, 603155 Nizhny Novgorod, Russia
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Issue 5
Keywords S. aureus
neutrophil heterogeneity
luminol-dependent chemiluminescence
ROS production
electrochemical detection
neutrophils
E. coli
nanoelectrodes
Language English
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Snippet The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical...
The reactive oxygen species (ROS) production by a single neutrophil after stimulation with and was estimated by an electrochemical amperometric method with a...
The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated by an electrochemical...
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StartPage 1361
SubjectTerms Analysis
B cells
Bacteria
Carbon
Cytochrome
Cytoplasm
E coli
electrochemical detection
Electrodes
Escherichia coli
Fibroblasts
Genotype & phenotype
Leukocytes (granulocytic)
Leukocytes (neutrophilic)
Methods
Morphology
neutrophil heterogeneity
Neutrophils
Population levels
Quantum dots
Reactive oxygen species
ROS production
S. aureus
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Title ROS Production by a Single Neutrophil Cell and Neutrophil Population upon Bacterial Stimulation
URI https://www.ncbi.nlm.nih.gov/pubmed/37239032
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Volume 11
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