Electrospun tilapia collagen nanofibers accelerating wound healing via inducing keratinocytes proliferation and differentiation

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 143; pp. 415 - 422
• Main Authors: Zhou, Tian, Wang, Nanping, Xue, Yang, Ding, Tingting, Liu, Xin, Mo, Xiumei, Sun, Jiao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2016
• Subjects: amino acids; Animals; Bandages; bioactive properties; biocompatible materials; Biomaterials; biomimetics; CD4-CD8 Ratio; CD4-Positive T-Lymphocytes - cytology; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - cytology; CD8-Positive T-Lymphocytes - immunology; Cell Differentiation - drug effects; Cell Line; Cell Proliferation - drug effects; collagen; Collagen - isolation & purification; Collagen - pharmacology; Collagens; colloids; contact angle; cost effectiveness; Dressing; Electrochemical Techniques; Electrospinning; Electrospun tilapia collagen nanofibers; extracellular matrix; Extracellular Matrix - chemistry; gene expression; Gene Expression Regulation - drug effects; HaCaTs differentiation; Humans; immunoglobulin G; immunoglobulin M; Intermediate Filament Proteins - genetics; Intermediate Filament Proteins - metabolism; keratinocytes; Keratinocytes - cytology; Keratinocytes - drug effects; lymphocytes; medicine; Mice; Mice, Inbred BALB C; Nanofibers; Nanofibers - chemistry; Nanofibers - therapeutic use; Protein Precursors - genetics; Protein Precursors - metabolism; protein-glutamine gamma-glutamyltransferase; Rat model; Rats; Rats, Sprague-Dawley; risk; Skin - drug effects; Skin - injuries; Skin - pathology; Skin wound healing; Sponges; Tensile Strength; thermal stability; Tilapia; Tilapia - metabolism; tissue repair; Transglutaminases - genetics; Transglutaminases - metabolism; Wound healing; Wound Healing - drug effects; HaCaTs differentiation; Rat model; Skin wound healing; Electrospun tilapia collagen nanofibers
• ISSN: 0927-7765; 1873-4367; 1873-4367
• DOI: 10.1016/j.colsurfb.2016.03.052

[Display omitted] •Collagen sponge from tilapia skin was extracted and proved to not induce obvious immune response.•Biomimetic electrospun tilapia collagen nanofibers with suitable tensile strength and thermal stability were developed.•The adhesion, proliferation and differentiation of HaCaTs were promoted by electrospun tilapia collagen nanofibers.•The electrospun tilapia collagen nanofibers could accelerate skin wound healing rapidly and effectively in the rat model. The development of biomaterials with the ability to induce skin wound healing is a great challenge in biomedicine. In this study, tilapia skin collagen sponge and electrospun nanofibers were developed for wound dressing. The collagen sponge was composed of at least two α-peptides. It did not change the number of spleen-derived lymphocytes in BALB/c mice, the ratio of CD4+/CD8+ lymphocytes, and the level of IgG or IgM in Sprague-Dawley rats. The tensile strength and contact angle of collagen nanofibers were 6.72±0.44MPa and 26.71±4.88°, respectively. They also had good thermal stability and swelling property. Furthermore, the nanofibers could significantly promote the proliferation of human keratinocytes (HaCaTs) and stimulate epidermal differentiation through the up-regulated gene expression of involucrin, filaggrin, and type I transglutaminase in HaCaTs. The collagen nanofibers could also facilitate rat skin regeneration. In the present study, electrospun biomimetic tilapia skin collagen nanofibers were succesfully prepared, were proved to have good bioactivity and could accelerate rat wound healing rapidly and effectively. These biological effects might be attributed to the biomimic extracellular matrix structure and the multiple amino acids of the collagen nanofibers. Therefore, the cost-efficient tilapia collagen nanofibers could be used as novel wound dressing, meanwhile effectively avoiding the risk of transmitting animal disease in the future clinical apllication.