A Simple Method for Predicting Modes of Actions of Antitumor Agents

• Published in: Journal of Pharmacobio-Dynamics Vol. 11; no. 4; pp. 241 - 244
• Main Authors: NAKAJIMA, Shokichi, FUKUYASU, Harumi, SATO, Yasumi, KAZUNO, Yuzo, YOSHIDA, Mitsuzi, HOSHI, Akio
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1988; Pharmaceutical Society of Japan
• Subjects: Adenosine - pharmacology; Animals; Antineoplastic agents; Antineoplastic Agents - pharmacology; Biological and medical sciences; DNA Replication - drug effects; General aspects; Guanosine - pharmacology; Leukemia L1210; Medical sciences; Mitosis - drug effects; Nucleosides - pharmacology; Pharmacology. Drug treatments; Protein Synthesis Inhibitors - pharmacology; RNA - antagonists & inhibitors; Thymidine - pharmacology; Tumor Cells, Cultured - drug effects; uridine; Uridine - pharmacology; Antineoplastic agent; Cell culture; RNA synthesis; Protein synthesis; Nucleoside; DNA synthesis; L1210-Leukemia; Inhibition; Mechanism of action; In vitro; Tumor cell
• ISSN: 0386-846X; 1881-1353
• DOI: 10.1248/bpb1978.11.241

Modes of actions of antitumor agents can be predicted by comparison of antitumor activities against L1210 cells in the presence and absence of some nucleosides. 1) Deoxyribonucleic acid (DNA) inhibitors were mainly antagonized by thymidine. 2) Both DNA and ribonucleic acid (RNA) inhibitors were mainly antagonized by adenosine and uridine. 3) Uridine antagonized the activities of an RNA inhibitor. 4) Nonc of these nucleosides tested antagonized the antitumor activities of an inhibitor of protein synthesis. 5) A mitotic inhibitor was weakly antagonized by thymidine, adenosine and uridine. This method is useful for approximating the modes of actions of antitumor agents without using radioactive precursors.