Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex

The Mycobacterium tuberculosis complex includes the four species M. tuberculosis, M. bovis, M. africanum, and M. microti. We sequenced 13 M. tuberculosis complex strains in the 16S-to-23S rDNA internal transcribed spacer (ITS). The ITS has a high rate of nucleotide substitution. Previous reports fou...

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Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 32; no. 7; pp. 1639 - 1643
Main Authors Frothingham, R, Hills, H.G, Wilson, K.H
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.07.1994
Subjects
Bacteriology
Base Sequence
Biological and medical sciences
Conserved Sequence
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Molecular Sequence Data
MYCOBACTERIUM
Mycobacterium - genetics
Mycobacterium bovis - genetics
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Phylogeny
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Genetic variability
Mycobacterium bovis
Nucleotide sequence
Ribosomal DNA
Homology
Spacer DNA
Phylogeny
Species complex
Mycobacterium tuberculosis
Mycobacteriales
Mycobacteriaceae
Bacteria
Actinomycetes
Mycobacterium microti
Mycobacterium africanum
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Summary:The Mycobacterium tuberculosis complex includes the four species M. tuberculosis, M. bovis, M. africanum, and M. microti. We sequenced 13 M. tuberculosis complex strains in the 16S-to-23S rDNA internal transcribed spacer (ITS). The ITS has a high rate of nucleotide substitution. Previous reports found three nucleotide substitutions in the ITS between two M. tuberculosis complex strains. In contrast, we found the same ITS sequence in all 13 M. tuberculosis complex strains (including all four species and M. bovis BCG). This finding confirms the conservation of 16S rDNA sequence and the high DNA-DNA relatedness found in previous studies. By the usual criteria, the four species of the M. tuberculosis complex would be considered a single species. In a phylogenetic analysis based on the ITS sequence, the four species of the M. tuberculosis complex were distinct from nontuberculous mycobacteria. The ITS contains at least seven potential sites for oligonucleotide probes with specificity for the M. tuberculosis complex
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ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.32.7.1639-1643.1994