Analysis of immune responses of different hosts to Babesia divergens isolates from different geographic areas and capacity of culture-derived exoantigens to induce efficient cross-protection

• Published in: Infection and Immunity Vol. 59; no. 8; pp. 2799 - 2805
• Main Authors: Precigout, E. (URA Centre National de la Recherche Scientifique, Poitiers, France), Gorenflot, A, Valentin, A, Bissuel, G, Carcy, B, Brasseur, P, Moreau, Y, Schrevel, J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.1991
• Subjects: Animals; Antibodies, Protozoan - immunology; ANTIGENE; ANTIGENOS; Antigens, Protozoan - immunology; Babesia - immunology; BABESIA DIVERGENS; Babesiosis - immunology; Babesiosis - prevention & control; Biological and medical sciences; BOVIN; Cattle; Cross Reactions; Detergents; Epitopes; Fundamental and applied biological sciences. Psychology; GANADO BOVINO; GENERO HUMANO; GENRE HUMAIN; GERBILLE; Gerbillinae; GERBO; Humans; Immunization; IMMUNSERUM; Life cycle. Host-agent relationship. Pathogenesis; MORTALIDAD; MORTALITE; Peptides - immunology; PODER PATOGENO; Polyethylene Glycols; POUVOIR PATHOGENE; Precipitin Tests; Protozoa; Protozoan Vaccines - administration & dosage; Protozoan Vaccines - immunology; REPONSE IMMUNITAIRE; RESPUESTA INMUNOLOGICA; Solubility; SUERO INMUNE; VACCINATION; VACUNACION; Human; Protozoa; Immunization; Protozoal disease; Immune response; Antibody; Cross protection; Babesia divergens; Infection; Antigen; Antiserum; Antigenicity; Animal; Isolate; Pathogen; Sporozoa; Humoral immunity
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.59.8.2799-2805.1991

The immunoprecipitation of [35S]methionine-radiolabelled antigens from different Babesia divergens isolates by using bovine, gerbil, and human immune sera has shown that many B. divergens proteins contain epitopes shared between isolates. The cross-protective capacity of culture-derived soluble immunogens from the B. divergens Rouen 1987 isolate was tested against different B. divergens isolates. Results showed complete protection against the 7107b French isolate and substantial protection against the Weybridge 8843 English isolate (80% protection) and the Munich 87 German isolate (60% protection). In order to explain these vaccination results and to assess both the common and variable antigenicity of B. divergens, the antigenic patterns of the challenge isolates (Rouen 1987, 7107b, Weybridge 8843, and Munich 87) were compared by immunoprecipitation, using gerbil antisera raised against the Rouen 1987 vaccine isolate. Differences in the antigenic patterns and in the cross-protection of gerbils in these heterologous challenges were examined by studying the virulence and the antigenic status of each isolate