Changes in annual transcriptome dynamics of a clone of Japanese cedar (Cryptomeria japonica D. Don) planted under different climate conditions

• Published in: PloS one Vol. 18; no. 2; p. e0277797
• Main Authors: Nose, Mine, Hanaoka, So, Fukatsu, Eitaro, Kurita, Manabu, Miura, Masahiro, Hiraoka, Yuichiro, Iki, Taiichi, Chigira, Osamu, Mishima, Kentaro, Takahashi, Makoto, Watanabe, Atsushi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.02.2023; Public Library of Science (PLoS)
• Subjects: Adaptation; Air temperature; Amino acids; Biology and Life Sciences; Cedar; Climate; Climatic conditions; Cloning; Cluster analysis; Clustering; Cold; Correlation coefficient; Correlation coefficients; Cryptomeria - physiology; Cryptomeria japonica; Day length; Environmental conditions; Gene expression; Genes; Least squares method; Nitrogen; Physical Sciences; Physiology; Plant growth; Principal components analysis; Regression analysis; Research and Analysis Methods; Seasons; Temperature; Transcriptome; Transcriptomes; Trees; Trees - physiology; Variance analysis; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0277797

Environmental responses are critical for plant growth and survival under different climate conditions. To elucidate the underlying biological mechanisms of environmental responses in Japanese cedar (Cryptomeria japonica D. Don), the annual transcriptome dynamics of common clonal trees (Godai1) planted at three different climate sites (Yamagata, Ibaraki, and Kumamoto Prefectures) were analyzed using microarrays. Both principal component analysis (PCA) and hierarchical clustering of the microarray data indicated the transition to dormant transcriptome status occurred earlier and the transition to active growth status later in the colder region. Interestingly, PCA also indicated that the transcriptomes of trees grown under three different conditions were similar during the growth period (June to September), whereas the transcriptomes differed between sites during the dormant period (January to March). In between-site comparisons, analyses of the annual expression profiles of genes for sites 'Yamagata vs. Kumamoto', 'Yamagata vs. Ibaraki', and 'Ibaraki vs. Kumamoto' identified 1,473, 1,137, and 925 targets exhibiting significantly different expression patterns, respectively. The total of 2,505 targets that exhibited significantly different expression patterns in all three comparisons may play important roles in enabling cuttings to adapt to local environmental conditions. Partial least-squares regression analysis and Pearson correlation coefficient analysis revealed that air temperature and day length were the dominant factors controlling the expression levels of these targets. GO and Pfam enrichment analyses indicated that these targets include genes that may contribute to environmental adaptation, such as genes related to stress and abiotic stimulus responses. This study provided fundamental information regarding transcripts that may play an important role in adaptation to environmental conditions at different planting sites.