Validation and measurement uncertainty evaluation of the GC-MS method for the direct determination of hexamethylenetetramine from foods

• Published in: Food science and biotechnology Vol. 25; no. 5; pp. 1305 - 1311
• Main Authors: Lim, Ho Soo, Hwang, Ju Young, Kim, Jung Im, Choi, Jae Chon, Kim, Meehye
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.10.2016; Springer Nature B.V; 한국식품과학회
• Subjects: Calibration; Chemistry; Chemistry and Materials Science; correlation; Correlation coefficient; Correlation coefficients; detection limit; Food; Food matrix; Food Science; foods; gas chromatography-mass spectrometry; Hexamethylenetetramine; Nutrition; quantitative analysis; Uncertainty; 식품과학; direct determination; method validation; gas chromatography-mass spectrometry; hexamethylenetetramine
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-016-0205-5

A simple GC-MS method has been developed and validated for the direct determination of hexamethylenetetramine (HMT). The separation of HMT was performed using a MXT-1 column. The calibration curve was linear over the concentration range 0.1-25 μg/mL, with a good correlation coefficient ( r =0.9996). The recoveries of HMT from foods spiked at 1, 5, and 10 μg/g ranged from 91.7% to 115.2%. Intra-day ( n =5) and inter-day ( n =5) precision were less than 7%. The limit of detection and the limit of quantification of the method were 0.05 and 0.15 μg/mL, respectively. The uncertainties associated with food matrix and calibration contributed most to the overall expanded uncertainty. The method validation data indicated that quantitative method could be applied to the direct determination of non-hydrolyzed HMT in foods.