Identification of hydatidosis-related modules and key regulatory genes

• Published in: PeerJ (San Francisco, CA) Vol. 8; p. e9280
• Main Authors: Song, Jijun, Song, Mingxin
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 18.06.2020; PeerJ, Inc; PeerJ Inc
• Subjects: Analysis; Apoptosis; Bone morphogenetic proteins; DNA binding proteins; Dogs; Echinococcosis; Echinococcus; Eggs; Gene expression; Genes; Host; Infections; Infectious diseases; Liver; Medical research; MicroRNA; miRNA; miRNA-184; Module; Nitrogen; Non-coding RNA; Parasites; Parasitology; Pathogenesis; Pathogenicity; Peptides; Physiological aspects; Proteins; Signal transduction; Transcription (Genetics); Transcription factors; Zoology; China; miRNA-184; Host; Module; Echinococcosis
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.9280

caused by larval of is prevalent all over the world. Although clinical experience showed that the presence of tapeworms could not be found in liver lesions, the repeated infection and aggravation of lesions still occur in the host. Here, this study constructed a multifactor-driven disease-related dysfunction network to explore the potential molecular pathogenesis mechanism in different hosts after infection. First, iTRAQ sequencing was performed on human liver infected with . Second, obtained microRNAs(miRNAs) expression profiles of humans and canine infected with from the GEO database. In addition, we also performed differential expression analysis, protein interaction network analysis, enrichment analysis, and crosstalk analysis to obtain genes and modules related to infection. Pivot analysis is used to calculate the potential regulatory effects of multiple factors on the module and identify related non-coding RNAs(ncRNAs) and transcription factors(TFs). Finally, we screened the target genes of miRNAs of to further explore its infection mechanism. A total of 267 differentially expressed proteins from humans and 3,635 differentially expressed genes from canine were obtained. They participated in 16 human-related dysfunction modules and five canine-related dysfunction modules, respectively. Both human and canine dysfunction modules are significantly involved in BMP signaling pathway and TGF-beta signaling pathway. In addition, pivot analysis found that 1,129 ncRNAs and 110 TFs significantly regulated human dysfunction modules, 158 ncRNAs and nine TFs significantly regulated canine dysfunction modules. Surprisingly, the miR-184 plays a role in the pathogenicity regulation by targeting nine TFs and one ncRNA in humans. Similarly, miR-184 can also cause physiological dysfunction by regulating two transcription factors in canine. The results show that the miRNA-184 of can regulate the pathogenic process through various biological functions and pathways. The results laid a solid theoretical foundation for biologists to further explore the pathogenic mechanism of .