cDNA cloning of squalene synthase genes from mono- and dicotyledonous plants, and expression of the gene in rice

• Published in: Plant and cell physiology Vol. 38; no. 12; pp. 1409 - 1413
• Main Authors: Hata, S. (Kyoto Univ. (Japan)), Sanmiya, K, Kouchi, H, Matsuoka, M, Yamamoto, N, Izui, K
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.12.1997
• Subjects: alkyl (aryl) transferases; Amino Acid Sequence; amino acid sequences; Biological and medical sciences; cell division; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; cloning; Cloning, Molecular; complementary DNA; DICOTILEDONEAS; DICOTYLEDONE; DICOTYLEDONS; DNA, Complementary; Enzymes; EXPRESION GENICA; EXPRESSION DES GENES; Farnesyl-Diphosphate Farnesyltransferase - genetics; Fundamental and applied biological sciences. Psychology; genbank/ab007501; genbank/ab007502; genbank/ab007503; GENE; GENE EXPRESSION; Gene Expression Regulation, Plant - radiation effects; GENES; Genes. Genome; genetic regulation; Glycine max; Glycine max - enzymology; Glycine max - genetics; gmss-7 genes; LIASAS; Light; LYASE; LYASES; Maize (Zea mays); messenger RNA; Metabolism; Molecular and cellular biology; MOLECULAR CLONING; Molecular genetics; Molecular Sequence Data; MONOCOTILEDONEAS; MONOCOTYLEDONE; MONOCOTYLEDONS; mRNA level; Oryza - enzymology; Oryza - genetics; Oryza - radiation effects; ORYZA SATIVA; osss-d genes; Phylogenetic tree; phylogeny; Plant physiology and development; Rice (Oriza sativa); Sequence Homology, Amino Acid; Soybeans (Glycine max); squalene; Squalene synthase (EC 2.5.1.21); Zea mays; Zea mays - enzymology; Zea mays - genetics; zmss-4 genes; Monocotyledones; Zea mays; Enzyme; Transferases; Phylogeny; Gene expression; Farnesyl-diphosphate farnesyltransferase; Glycine max; Cereal crop; Grain legume; Oryza sativa; Regulation(control); Leguminosae; Complementary DNA; Gramineae; Dicotyledones; Light; Angiospermae; Isolation; Spermatophyta; Aminoacid sequence
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029137

cDNA clones encoding squalene synthases were isolated from rice, maize and soybeans. A phylogenetic tree showed that the enzymes of monocots and dicots form distinct subgroups. In rice, squalene synthase mRNA was detected in tissues containing dividing cells and its level was repressed by illumination.