Modulation of stress response and productive performance of Litopenaeus vannamei through diet

• Published in: PeerJ (San Francisco, CA) Vol. 7; p. e6850
• Main Authors: Martínez-Antonio, Eliza M, Racotta, Ilie S, Ruvalcaba-Márquez, Juan C, Magallón-Barajas, Francisco
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 08.05.2019; PeerJ, Inc; PeerJ Inc
• Subjects: Amino acids; Aquaculture; Aquaculture, Fisheries and Fish Science; Arginine; Biochemistry; Carbohydrates; Chronic and acute stressors; Decapoda; Diet; Dietary minerals; Energy; Energy balance; Energy charge; Escape response; Fish; Gene expression; Glucose; Hemolymph; Hepatopancreas; High carbohydrate diet; High protein diet; Hypoxia; Laboratories; Lactates; Lactic acid; Lipids; Litopenaeus vannamei; Low carbohydrate diet; Low protein diet; Low salinity; Marine Biology; Metabolism; Nutrient deficiency; Nutrition research; Phosphates; Physiology; Pollution; Probiotics; Proteins; Salinity; Salinity effects; Shellfish; Stress (Psychology); Stress response; Water pollution; Mexico; Hypoxia; Diet; Low salinity; Escape response; Chronic and acute stressors
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.6850

The high tolerance of to a wide range of salinity (1-50 psu) makes this species an excellent candidate for culture under low salinity, decreasing shrimp epidemics and water pollution in some coastal areas. However, salinity levels outside the optimal range could impose several physiological constraints that would in turn affect growth and survival, particularly in the presence of additional stressors (e.g. high densities, handling practices, and hypoxia). Despite shrimp susceptibility to individual stressors has been widely addressed, information regarding response to chronic and acute stressors combined and its relation to diet is scarce. Thus, the aim of our study was to determine the effect of diet on the susceptibility to chronic (low salinity) and acute (hypoxia and escape response) stressors in terms of culture performance and physiological indicators. We evaluated overall performance during culture of at low salinity (6 psu), fed with an experimental diet with low protein and high carbohydrate content (26% protein and 6% fish meal plus probiotic mixture) and compared to a commercial formula with high protein and low carbohydrate content (40% crude protein and 20% fish meal without probiotic mixture). At the end of the rearing experiment, shrimp were exposed to two types of acute stress, hypoxia and escape. Biochemical (hemocyanin, total proteins, glucose, and lactate) and bioenergetic (adenylic energy charge and arginine phosphate levels) variables were measured to assess chronic stress response (salinity) and acute stress response (hypoxia or escape). The experimental diet resulted in higher muscle energy status that was not affected by low salinity, although lipid levels were lower under this condition. This diet partially counteracted the low performance at low salinity and promoted greater protein efficiency. Hypoxia induced strong hyperglycemic and lactate increase as response, whereas escape response was characterized by a depletion of arginine phosphate levels, with a stronger decrease in shrimp fed experimental diet, due to the high initial level of this reserve. Some data (glucose levels in hemolymph and lipids in hepatopancreas) suggest that shrimp under chronic stress conditions (low salinity and high densities) present a low ability to respond to subsequent acute stressors such as hypoxia or escape. This work indicates that diet can increase the energy status of shrimp, enabling them to overcome potential multifactorial stressors, which are common in farming systems.