Molecular genetic analysis of beta-toxin of Clostridium perfringens reveals sequence homology with alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus

Oligonucleotide probes designed on the basis of the N-terminal sequence of Clostridium perfringens beta-toxin were used to isolate the encoding gene (cpb). The nucleotide sequence of cpb was determined, and on the basis of DNA hybridization experiments it was shown that the gene is found only in typ...

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Published inInfection and Immunity Vol. 61; no. 9; pp. 3958 - 3965
Main Authors Hunter, S.E.C, Brown, J.E, Oyston, P.C.F, Sakurai, J, Titball, R.W
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.09.1993
Subjects
Amino Acid Sequence
Bacterial Proteins
Bacterial Toxins - biosynthesis
Bacterial Toxins - chemistry
Bacterial Toxins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Cloning, Molecular
CLOSTRIDIUM PERFRINGENS
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Hemolysin Proteins - chemistry
Leukocidins - chemistry
Microbiology
Molecular Sequence Data
Oligonucleotide Probes
Open Reading Frames
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
STAPHYLOCOCCUS AUREUS
Staphylococcus aureus
Nucleotide sequence
Sequence alignment
Clostridiales
Homology
Clostridium perfringens
Toxin
Gene
Clostridiaceae
N terminal-Sequence
Bacteria
Micrococcales
Micrococcaceae
Comparative study
Staphylococcus aureus
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Summary:Oligonucleotide probes designed on the basis of the N-terminal sequence of Clostridium perfringens beta-toxin were used to isolate the encoding gene (cpb). The nucleotide sequence of cpb was determined, and on the basis of DNA hybridization experiments it was shown that the gene is found only in type B and C strains of C. perfringens. The deduced amino acid sequence of the beta-toxin revealed homology with the alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus. The beta-toxin purified from C. perfringens appeared to exist in monomeric and multimeiric forms. Recombinant beta-toxin, produced in Escherichia coli, appeared to be mainly in the multimeric form
Bibliography:L73
9430485
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ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.61.9.3958-3965.1993