The Role of Oxidative DNA Damage in Human Arsenic Carcinogenesis: Detection of 8-Hydroxy-2′-Deoxyguanosine in Arsenic-Related Bowen's Disease
Arsenic is widely distributed in nature in the form of either metalloids or chemical compounds, which cause a variety of pathologic conditions including cutaneous and visceral malignancies. Recently, reactive oxygen species have been hypothesized to be one of the causes of arsenic-induced carcinogen...
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Published in | Journal of investigative dermatology Vol. 113; no. 1; pp. 26 - 31 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Danvers, MA
Elsevier Inc
01.07.1999
Nature Publishing |
Subjects | |
Online Access | Get full text |
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Summary: | Arsenic is widely distributed in nature in the form of either metalloids or chemical compounds, which cause a variety of pathologic conditions including cutaneous and visceral malignancies. Recently, reactive oxygen species have been hypothesized to be one of the causes of arsenic-induced carcinogenesis. 8-Hydroxy-2′-deoxyguanosine is one of the major reactive oxygen species-induced DNA base-modified products that is widely accepted as a sensitive marker of oxidative DNA damage. We studied the presence of 8-hydroxy-2′-deoxyguanosine by immunohistochemistry using N45.1 monoclonal antibody in 28 cases of arsenic-related skin neoplasms and arsenic keratosis as well as in 11 cases of arsenic-unrelated Bowen’s diseases. The frequency of 8-hydroxy-2′-deoxyguanosine positive cases was significantly higher in arsenic-related skin neoplasms (22 of 28; 78%) than in arsenic-unrelated Bowen’s disease (one of 11; 9%) (p < 0.001 by χ2 test). 8-Hydroxy-2′-deoxyguanosine was also detected in normal tissue adjacent to the arsenic-related Bowen’s disease lesions. Furthermore, arsenic was detected by neutron activation analysis in the deparaffined skin tumor samples of arsenic-related disease (four of five; 80%), whereas arsenic was not detected in control samples. Our results strongly suggest the involvement of reactive oxygen species in arsenic-induced human skin cancer. Key word: neutron activation analysis. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0022-202X 1523-1747 |
DOI: | 10.1046/j.1523-1747.1999.00630.x |