AIF1 was identified as an up-regulated gene contributing to CSFV Shimen infection in porcine alveolar macrophage 3D4/21 cells

• Published in: PeerJ (San Francisco, CA) Vol. 8; p. e8543
• Main Authors: Gong, Xiaocheng, Li, Xuepeng, You, Xin, Hu, Aoxue, Liu, Min, Yao, Huimin, He, Jun, Zhang, Xianghan, Ning, Pengbo
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 17.02.2020; PeerJ, Inc; PeerJ Inc
• Subjects: Agricultural Science; AIF1; Alveoli; Cell activation; Cell adhesion & migration; Cloning; CSFV Shimen; Diseases; Fever; Gene expression; Hog cholera; IL6; Infection; Infections; Inflammation; Interleukin 6; Macrophages; Messenger RNA; Microscopy; Plasmids; Polymerase chain reaction; Porcine alveolar macrophages; Proteins; Replication; RNA; Software; Statistical analysis; Swine; Time; Transcription; Transplantation; Transplants & implants; Veterinary Medicine; Viral infections; Virology; Viruses; China; CSFV Shimen; Porcine alveolar macrophages; IL6; AIF1
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.8543

Classical swine fever (CSF) is a disease that is characterized by diffuse hemorrhaging, high fever, and high mortality rates. The pro-inflammatory characteristics of allograft inflammatory factor 1 (AIF1) have been well documented; however, insufficient attention has been given to porcine AIF1. In the present study, AIF1 was identified as a key player contributing to CSFV Shimen infection in porcine alveolar macrophage (PAM) 3D4/21 cell line. Our evaluation showed that AIF1 mRNA and protein are expressed at a time-dependent high level in CSFV Shimen-infected PAM 3D4/21 cells. The transcription and translation of IL6 were also significantly upregulated in infected PAM 3D4/21 cells. By utilizing overexpression RNAs approach, we showed that the cellular AIF1 induced an increased IL6 in PAM 3D4/21 cells. Furthermore, silencing of AIF1 suppressed CSFV Shimen-induced IL6 production in PAM 3D4/21 cells and also inhibited CSFV replication, whereas overexpression of recombinant AIF1 was beneficial for the replication of CSFV Shimen and promoting IL6 production in CSFV Shimen-infected PAM 3D4/21 cells. It is suggested CSFV Shimen induced IL6 in PAM 3D4/21 cells via AIF1 activation, which help clarify the AIF1-related inflammatory processes that occur on CSFV Shimen infected macrophages.