Solubilization of insoluble inorganic phosphates by a novel salt- and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere
To develop environment-friendly biofertilizer solubilizing insoluble phosphates, salt- and pH-tolerant, insoluble inorganic phosphate-solubilizing bacterium was isolated from soybean rhizosphere. On the basis of its physiological characteristics and Vitek analysis, this bacterium was identified as P...
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Published in | Bioresource technology Vol. 97; no. 2; pp. 204 - 210 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
2006
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | To develop environment-friendly biofertilizer solubilizing insoluble phosphates, salt- and pH-tolerant, insoluble inorganic phosphate-solubilizing bacterium was isolated from soybean rhizosphere. On the basis of its physiological characteristics and Vitek analysis, this bacterium was identified as
Pantoea agglomerans. The optimal medium composition and cultural conditions for the solubilization of insoluble phosphate by
P. agglomerans R-42 were 3% (w/v) of glucose, 0.1% (w/v) of NH
4NO
3, 0.02% (w/v) of MgSO
4
·
7H
2O, and 0.06% (w/v) of CaCl
2
·
2H
2O along with initial pH 7.5 at 30
°C. The soluble phosphate production under optimal condition was around 900
mg/l, which was approximately 4.6-fold higher than the yield in the MPVK medium. The solubilization of insoluble phosphate was associated with a drop in the pH of the culture medium.
P. agglomerans R-42 showed resistance against different environmental stresses like 5–45
°C temperature, 1–5% salt concentration and 3–11 pH range. Insoluble phosphate solubilization was highest from CaHPO
4 (1367
mg/l), hydroxyapatite (1357
mg/l) and Ca
3(PO
4)
2 (1312
mg/l). However, the strain produced soluble phosphate to the culture broth with the concentrations of 28
mg/l against FePO
4, and 19
mg/l against AlPO
4, respectively. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2005.02.021 |