Oral immunization with HpaA affords therapeutic protective immunity against H. pylori that is reflected by specific mucosal immune responses

• Published in: Vaccine Vol. 25; no. 14; pp. 2591 - 2598
• Main Authors: Nyström, Johanna, Svennerholm, Ann-Mari
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 30.03.2007; Elsevier
• Subjects: Adhesins; Adhesins, Bacterial - immunology; Administration; Administration, Oral; Allergy and Immunology; Animals; Antibodies; Antibodies, Bacterial - biosynthesis; Applied microbiology; Bacterial; Bacterial Vaccines; Bacterial Vaccines - immunology; Biological and medical sciences; biosynthesis; Cytokines; Cytokines - biosynthesis; Female; Fundamental and applied biological sciences. Psychology; H. pylori; Helicobacter Infections; Helicobacter Infections - prevention & control; Helicobacter pylori; Helicobacter pylori - immunology; HpaA; Immunity; Immunity, Mucosal; Immunization; Immunoglobulin A; Immunoglobulin A, Secretory - biosynthesis; immunology; Inbred C57BL; Mice; Mice, Inbred C57BL; Microbiology; Microbiology in the Medical Area; Mikrobiologi inom det medicinska området; Mucosal; Mucosal immune responses; Oral; prevention & control; Secretory; Stomach; Stomach - microbiology; Therapeutic immunization; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); H. pylori; HpaA; Mice; Mucosal immune responses; Therapeutic immunization; Immunoprotection; Immunization; Immune response; Mucosa; Rodentia; Vaccine; Vertebrata; Mammalia; Mouse
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2006.12.026

In the present study, we evaluated the capacity of Helicobacter pylori adhesin A (HpaA), a H. pylori specific colonization factor, to induce therapeutic protection against H. pylori infection in mice. We found that oral immunization of H. pylori infected mice with HpaA induced protection, i.e. significant reduction in bacterial load in the stomach. This was even more pronounced when a combination of HpaA and urease was used. The protection was strongly related to specific mucosal CD4 + T cell responses with a Th1 profile as well as to mucosal IgA responses locally in the stomach. These findings suggest that HpaA is a promising vaccine candidate antigen for use in a therapeutic vaccine against H. pylori.