Oryza sativa Brittle Culm 1-like 6 modulates β-glucan levels in the endosperm cell wall

• Published in: PloS one Vol. 14; no. 5; p. e0217212
• Main Authors: Midorikawa, Keiko, Kuroda, Masaharu, Yamashita, Haruyuki, Tamura, Tomoko, Abe, Keiko, Asakura, Tomiko
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.05.2019; Public Library of Science (PLoS)
• Subjects: Agriculture; Analogs; beta-Glucans - metabolism; Binding sites; Biology and Life Sciences; Biosynthesis; Brittleness; Carbohydrates; Cell fusion; Cell Wall - metabolism; Cell walls; Cellulose; Cereals; Endosperm; Endosperm - genetics; Endosperm - growth & development; Endosperm - metabolism; Enlargement; Fragility; Fusion protein; Gene expression; Gene Expression Regulation, Plant; Genomics; Glucan; Grain; Hair; Life sciences; Ligands; Molecular machines; Morphogenesis; mRNA; Nitrogen; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Physical Sciences; Plant Proteins - genetics; Plant Proteins - metabolism; Protein biosynthesis; Protein transport; Proteins; Research and Analysis Methods; Rice; Ripening; RNA-mediated interference; Seeds; Seeds - genetics; Seeds - growth & development; Seeds - metabolism; Surface plasmon resonance; Transcription; Water absorption; β-Glucan; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217212

The endosperm cell wall affects post-harvest grain quality by affecting the mechanical fragility and water absorption of the grain. Therefore, understanding the mechanism underlying endosperm cell wall synthesis is important for determining the growth and quality of cereals. However, the molecular machinery mediating endosperm cell wall biosynthesis is not well understood. In this study, we investigated the role of Oryza sativa Brittle Culm 1-like 6 (OsBC1L6), a member of the COBRA-like protein family, in cellulose synthesis in rice. OsBC1L6 mRNA was expressed in ripening seeds during endosperm enlargement. When OsBC1L6-RFP was expressed in Arabidopsis cell cultures, this fusion protein was transported to the plasma membrane. To investigate the target molecules of OsBC1L6, we analyzed the binding interactions of OsBC1L6 with cellohexaose and the analogs using surface plasmon resonance, determining that cellohexaose bound to OsBC1L6. The β-glucan contents were significantly reduced in OsBC1L6-RNAi calli and OsBC1L6-deficient seeds from a Tos insertion mutant, compared to their wild-type counterparts. These findings suggest that OsBC1L6 modulates β-glucan synthesis during endosperm cell wall formation by interacting with cellulose moieties on the plasma membrane during seed ripening.