Aging impairs osteoblast differentiation of mesenchymal stem cells grown on titanium by favoring adipogenesis

We investigated the osteoblast and adipocyte differentiation of mesenchymal stem cells (MSCs) from young and aged rats cultured on Ti. Bone marrow MSCs derived from 1-month and 21-month rats were cultured on Ti discs under osteogenic conditions for periods of up to 21 days and osteoblast and adipocy...

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Published inJournal of applied oral science Vol. 24; no. 4; pp. 376 - 382
Main Authors Abuna, Rodrigo Paolo Flores, Stringhetta-Garcia, Camila Tami, Fiori, Leonardo Pimentel, Dornelles, Rita Cassia Menegati, Rosa, Adalberto Luiz, Beloti, Marcio Mateus
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Faculdade De Odontologia De Bauru - USP 01.07.2016
University of São Paulo
Subjects
Adipocytes
Adipogenesis - physiology
Age Factors
Aging
Aging - physiology
Alkaline Phosphatase - analysis
Animals
Cell Proliferation - physiology
Cells, Cultured
Dental Implants
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Female
Gene Expression
Lipids - analysis
Mesenchymal Stromal Cells - physiology
Original
Osteoblasts
Osteoblasts - physiology
Osteogenesis - physiology
Rats
Real-Time Polymerase Chain Reaction
Stem cells
Surface Properties
Titanium
Titanium - chemistry
Aging
Titanium
Adipocytes
Dental implants
Osteoblasts
Stem cells
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Summary:We investigated the osteoblast and adipocyte differentiation of mesenchymal stem cells (MSCs) from young and aged rats cultured on Ti. Bone marrow MSCs derived from 1-month and 21-month rats were cultured on Ti discs under osteogenic conditions for periods of up to 21 days and osteoblast and adipocyte markers were evaluated. Cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization and gene expression of RUNX2, osterix, ALP, bone sialoprotein, osteopontin, and osteocalcin were reduced in cultures of 21-month rats compared with 1-month rats grown on Ti. Gene expression of PPAR-γ , adipocyte protein 2, and resistin and lipid accumulation were increased in cultures of 21-month rats compared with 1-month rats grown on the same conditions. These results indicate that the lower osteogenic potential of MSCs derived from aged rats compared with young rats goes along with the higher adipogenic potential in cultures grown on Ti surface. This unbalance between osteoblast and adipocyte differentiation should be considered in dental implant therapy to the elderly population.
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All authors confirm that there is no conflict of interest associated with this study and there has been no financial support that could have influenced our results.
Disclosure statement
ISSN:1678-7757
1678-7765
1678-7765
1678-7757
DOI:10.1590/1678-775720160037