Effect of spleen cell populations on resolution of Cryptosporidium parvum infection in SCID mice

• Published in: Infection and Immunity Vol. 62; no. 4; pp. 1474 - 1477
• Main Authors: Perryman, L.E, Mason, P.H, Chrisp, C.E
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.1994
• Subjects: Animals; Biological and medical sciences; CD4-Positive T-Lymphocytes - physiology; CD8 Antigens - analysis; Cryptosporidiosis - immunology; CRYPTOSPORIDIUM; Cryptosporidium parvum; DEFICIENCE IMMUNOLOGIQUE; DEFICIENCIA INMUNOLOGICA; Fundamental and applied biological sciences. Psychology; Invertebrates; Life cycle. Host-agent relationship. Pathogenesis; LINFOCITOS; LYMPHOCYTE; Male; Mice; Mice, Inbred BALB C; Mice, SCID; Protozoa; RATON; SOURIS; Spleen - cytology; Spleen - immunology; T-Lymphocytes - physiology; T-Lymphocytes - transplantation; Protozoa; Protozoal disease; Rodentia; Host agent relation; Cell subpopulation; Infection; Cryptosporidiosis; Resistance; Vertebrata; Mammalia; Cryptosporidium parvum; Mouse; Persistent infection; Pathogenic; T-Lymphocyte; Passive transfer; Severe; Combined immune deficiency; Sporozoa
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.62.4.1474-1477.1994

Persistent infection was established in SCID mice given 10(7) Cryptosporidium parvum oocysts. Nine groups of infected SCID mice were inoculated with 10(6), 10(5), or 10(4) total spleen cells, CD8-depleted spleen cells, or CD4-depleted spleen cells from naive BALB/c donors. Infection was significantly reduced in all treatment groups. The most profound effect occurred with spleen cell preparations containing CD4 T lymphocytes but depleted of CD8 T lymphocytes