Mapping of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms

• Published in: Crop science Vol. 38; no. 3; pp. 843 - 850
• Main Authors: Spaner, D. (McGill University, Ste- Anne-de-Bellevue, QC, Canada.), Shugar, L.P, Choo, T.M, Falak, I, Briggs, K.G, Legge, W.G, Falk, D.E, Ullrich, S.E, Tinker, N.A, Steffenson, B.J
• Format: Journal Article
• Language: English
• Published: Madison, WI Crop Science Society of America 01.05.1998; American Society of Agronomy
• Subjects: Agronomy. Soil science and plant productions; Airborne microorganisms; ALBERTA; ALLELES; Biological and medical sciences; CARTE GENETIQUE; CHROMOSOME; CHROMOSOME MAPS; CHROMOSOMES; CROMOSOMAS; CROSSBREDS; DEVELOPMENTAL STAGES; Disease; DISEASE RESISTANCE; ENFERMEDADES FUNGOSAS; ERYSIPHE GRAMINIS; ERYSIPHE GRAMINIS F.SP. HORDEI; ETAPAS DE DESARROLLO; FENOTIPOS; Fundamental and applied biological sciences. Psychology; FUNGAL DISEASES; GENE; GENES; GENETIC MAPPING; GENETIC MAPS; GENETIC MARKERS; GENETIC RESISTANCE; Genetics; Genetics and breeding of economic plants; Grain; HORDEUM VULGARE; ILE DU PRINCE EDOUARD; ISLA DEL PRINCIPE EDUARDO; LOCI; LOCUS; MADURACION; MALADIE FONGIQUE; MANITOBA; MAPAS GENETICOS; MARCADORES GENETICOS; MARQUEUR GENETIQUE; MATURATION; ONTARIO; PATHOGENICITY; PATOGENICIDAD; Pest resistance; PHENOTYPE; PHENOTYPES; Plant pathogens; POUVOIR PATHOGENE; PRINCE EDWARD ISLAND; PRODUCTOS DEL CRUZAMIENTO; PRODUIT DE CROISEMENT; PUCCINIA GRAMINIS; PUCCINIA HORDEI; PYRENOPHORA TERES; RESISTANCE AUX MALADIES; RESISTANCE GENETIQUE; RESISTENCIA A LA ENFERMEDAD; RESISTENCIA GENETICA; RHYNCHOSPORIUM SECALIS; ROUILLE; ROYA; RUSTS; SIMPLE INTERVAL MAPPING; SINTOMAS; STADE DE DEVELOPPEMENT; SYMPTOME; SYMPTOMS; Varietal selection. Specialized plant breeding, plant breeding aims; VIRULENCE; WASHINGTON; Puccinia graminis f. tritici; Monocotyledones; Plant pathogen; Pyrenophora teres; Basidiomycetes; Cereal crop; Fungi; Disease resistance; Gramineae; Angiospermae; Ascomycetes; Erysiphe graminis f. hordei; Fungi Imperfecti; Locus; Thallophyta; Genetic mapping; Agronomical character; Rhynchosporium secalis; Hordeum vulgare; Visual observation; Host agent relation; Quantitative trait loci; Sensitivity resistance; Restriction fragment length polymorphism; Spermatophyta
• ISSN: 0011-183X; 1435-0653
• DOI: 10.2135/cropsci1998.0011183x003800030037x

Using field-scored data of disease severity under natural infestation, we mapped loci affecting resistance to powdery mildew (Blumeria graminis DC f. sp. hordei Em. Marchal), leaf rust (Puccinia hordei Otth.), stem rust (Puccinia graminis f. sp. tritici Eriks. and E. Henn.), scald [Rhynchosporium secalis (Oudem.) J.J. Davis], and net blotch (Pyrenophora teres Drechs.). The mapping population included parents and doubled-haploid progeny of the two-row barley cross Harrington/TR306. Resistance was affected by two to five loci, explaining 8 to 45% of the phenotypic variance, per disease. All chromosomes, except chromosome 5 (1H), contained regions with at least one disease resistance locus. One region on chromosome 4 (4H) contributed to resistance to stem rust, scald, and net blotch. This region has previously been reported to affect days to heading and maturity. Two known resistance genes in the population, Rpg1 and Mlg, were mapped to within 3 centimorgans (cM) of their previously estimated genomic locations by simple interval mapping of the field-scored data. This indicates that the genomic positions of disease resistance genes can be estimated accurately with simple interval mapping, even on the basis of field-scored data