Perisomatic innervation and neurochemical features of giant pyramidal neurons in both hemispheres of the human primary motor cortex

• Published in: Brain Structure and Function Vol. 226; no. 1; pp. 281 - 296
• Main Authors: Szocsics Péter, Papp Péter, Havas László, Watanabe, Masahiko, Maglóczky Zsófia
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Science and Business Media LLC 01.01.2021; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Adult; Aged; Amino acids; Biomedical and Life Sciences; Biomedicine; Calcium-binding protein; Cell Biology; Cortex (motor); Electron microscopy; Female; Forensic science; Glutamic acid transporter; Humans; Innervation; Interneurons; Localization; Male; Middle Aged; Motor Cortex; Neurological diseases; Neurology; neurológia; Neurosciences; Original; Original Article; Parvalbumin; Parvalbumins; Presynaptic Terminals; pszichiátria; Pyramidal Cells; QD Chemistry / kémia; RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan; Vesicular inhibitory amino acid transporter; Human; Primary motor cortex; Neurochemical features; Innervation; Betz cells; Post-mortem; Parvalbumin
• ISSN: 1863-2653; 1863-2661; 1863-2661; 0340-2061
• DOI: 10.1007/s00429-020-02182-8

Betz cells—the gigantopyramidal neurons found in high amount in the primary motor cortex—are among of the most characteristic neuronal cells. A part of them contains the calcium-binding protein parvalbumin (PV) in primates. However, less is known about these cells in the human motor cortex despite their important role in different neurological disorders. Therefore, the aim of our study was to investigate the neurochemical features and perisomatic input properties of Betz cells in control human samples with short post-mortem interval. We used different microscopic techniques to investigate the primary motor cortex of both hemispheres. The soma size and density, and expression of PV of the Betz cells were investigated. Furthermore, we used confocal fluorescent and electron microscopy to examine their perisomatic input. The soma size and density showed moderate variability among samples and hemispheres. Post-mortem interval and hemispherical localization did not influence these features. Around 70% of Betz cells expressed PV, but in less intensity than the cortical interneurons. Betz neurons receive dense perisomatic input, which are mostly VIAAT- (vesicular inhibitory amino acid transporter) and PV immunopositive. In the electron microscope, we found PV-immunolabelled terminals with asymmetric-like synaptic structure, too. Terminals with morphologically similar synaptic specialisation were also found among vGluT2- (vesicular glutamate transporter type 2) immunostained terminals contacting Betz cells. Our data suggest that Betz cells’ morphological properties showed less variability among subjects and hemispheres than the density of them. Their neurochemical and perisomatic input characteristics support their role in execution of fast and precise movements.