d-Fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode

• Published in: Biosensors & bioelectronics Vol. 24; no. 5; pp. 1184 - 1188
• Main Authors: Tominaga, Masato, Nomura, Shinya, Taniguchi, Isao
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier B.V 2009; Elsevier
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biotechnology; Carbon nanotube; Catalytic current; Electrochemistry - instrumentation; Electrochemistry - methods; Electron transfer reaction; Electron Transport; Enzymes, Immobilized - chemistry; Equipment Design; Equipment Failure Analysis; Fructose; Fructose - analysis; Fructose - chemistry; Fructose dehydrogenase; Fundamental and applied biological sciences. Psychology; Microelectrodes; Nanotechnology - instrumentation; Nanotechnology - methods; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - ultrastructure; Oxidoreductases - chemistry; Platinum - chemistry; Reproducibility of Results; Sensitivity and Specificity; Carbon nanotube; Catalytic current; Fructose dehydrogenase; Electron transfer reaction; Fructose; Enzyme; Nanotube; Electron transfer; Dehydrogenase; Carbon; Electrodes; Platinum; Oxidoreductases; Detection
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2008.07.002

Multi-walled carbon nanotubes (MWCNTs) were synthesized on platinum plate electrodes by the chemical vapor deposition (CVD) method. From the results of X-ray photoelectron spectroscopy and voltammetric investigation, the iron nanoparticles used as a catalyst for the MWCNT synthesis were enclosed with MWCNTs. The MWCNTs synthesized on the Pt plate (MWCNTs/Pt) electrode were immediately immersed into solutions of d-fructose dehydrogenase (FDH) to immobilize the enzyme onto the MWCNTs/Pt electrode surfaces. After the FDH was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current based on FDH was observed from ca. −0.15 V (versus Ag/AgCl/sat’d KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus substrate, the calibration range for the fructose concentration was up to ca. 40 mmol dm −3, and the apparent Michaelis–Menten constant was evaluated to be 11 ± 1 mmol dm −3.