Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis

• Published in: Cell research Vol. 21; no. 11; pp. 1551 - 1563
• Main Authors: Wang, Yu-Chieh, Nakagawa, Masato, Garitaonandia, Ibon, Slavin, Ileana, Altun, Gulsah, Lacharite, Robert M, Nazor, Kristopher L, Tran, Ha T, Lynch, Candace L, Leonardo, Trevor R, Liu, Ying, Peterson, Suzanne E, Laurent, Louise C, Yamanaka, Shinya, Loring, Jeanne F
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.11.2011; Nature Publishing Group
• Subjects: 631/136/2444; 631/136/532/2064; 631/45/612/1235; 631/80/458/1524; biomarkers; Biomarkers - metabolism; Biomedical and Life Sciences; Biotin - chemistry; Biotin - metabolism; Cell Biology; Cell culture; Cell Separation; Cells, Cultured; Embryo cells; Embryonic Stem Cells - cytology; Flow cytometry; Fluorescence; Fluorescence microscopy; Fucosyltransferases - metabolism; Gene expression; Gene Expression Profiling; Glycomics; Glycoproteins; Glycosylation; Humans; Induced Pluripotent Stem Cells - cytology; Lectins; Lectins - chemistry; Lectins - metabolism; Life Sciences; Neolactotetraosylceramide alpha -2,3-sialyltransferase; Original; original-article; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Protein Array Analysis; Protein Binding; Quality control; Sialyltransferases - metabolism; Somatic cells; Stem cells; 人类胚胎干细胞; 基因表达分析; 外源凝集素; 多能干细胞; 生物标志物; 细胞分离; 细胞类型; 阵列; glycosylation; induced pluripotent stem cells; human pluripotent stem cell; embryonic stem cells; lectins; pluripotency biomarkers
• ISSN: 1001-0602; 1748-7838; 1748-7838
• DOI: 10.1038/cr.2011.148

Rapid and dependable methods for isolating human pluripotent stem cell （hPSC） populations are urgently needed for quality control in basic research and in cell-based therapy applications. Using lectin arrays, we analyzed glyco- proteins extracted from 26 hPSC samples and 22 differentiated cell samples, and identified a small group of lectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types. These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined, regardless of the laboratory of origin, the culture conditions, the somatic cell type reprogrammed, or the reprogramming method used. We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluores- cence microscopy and flow cytometry, and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation. Global gene expression analysis showed pluripotency-associated differential expres- sion of specific fucosyltransferases and sialyltransferases, which may underlie these differences in protein glycosy- lation and lectin binding. Taken together, our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells, and demonstrate that lectins may be used as biomarkers to monitor pluripo- tency in stem cell populations and for removal of viable hPSCs from mixed cell population