Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977

The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protop...

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Published inBioscience, biotechnology, and biochemistry Vol. 67; no. 6; pp. 1434 - 1437
Main Authors Moriya, T. (Meiji Seika Kaisha Ltd., Odawara, Kanagawa (Japan). Pharmaceutical Technology Labs.), Watanabe, M, Sumida, N, Okakura, K, Murakami, T
Format Journal Article
LanguageEnglish
Published Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2003
Japan Society for Bioscience Biotechnology and Agrochemistry
Oxford University Press
Subjects
avi2
Biological and medical sciences
CELLULASE
Cellulases - biosynthesis
Cellulases - genetics
Cellulases - metabolism
Cloning, Molecular
DNA, Fungal
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genetic Vectors
HUMICOLA
Humicola insolens
Mitosporic Fungi - genetics
MOLECULAR CLONING
NUCLEOTIDE SEQUENCE
protoplast
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transformation, Genetic
TRANSGENICS
Enzyme
Gene overexpression
protoplast
Humicola insolens
Humicola
Fungi
avi2
Cellulase
Glycosidases
Hydrolases
Fungi Imperfecti
O-Glycosidases
Thallophyta
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Abstract The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.
AbstractList The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.
Author Murakami, T
Okakura, K
Watanabe, M
Sumida, N
Moriya, T. (Meiji Seika Kaisha Ltd., Odawara, Kanagawa (Japan). Pharmaceutical Technology Labs.)
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Cites_doi 10.1007/978-94-011-2930-5_2
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Keywords Enzyme
Gene overexpression
protoplast
Humicola insolens
Humicola
Fungi
avi2
Cellulase
Glycosidases
Hydrolases
Fungi Imperfecti
O-Glycosidases
Thallophyta
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Snippet The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with...
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SubjectTerms avi2
Biological and medical sciences
CELLULASE
Cellulases - biosynthesis
Cellulases - genetics
Cellulases - metabolism
Cloning, Molecular
DNA, Fungal
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genetic Vectors
HUMICOLA
Humicola insolens
Mitosporic Fungi - genetics
MOLECULAR CLONING
NUCLEOTIDE SEQUENCE
protoplast
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transformation, Genetic
TRANSGENICS
Title Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977
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Volume 67
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