Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977

The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protop...

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Published inBioscience, biotechnology, and biochemistry Vol. 67; no. 6; pp. 1434 - 1437
Main Authors Moriya, T. (Meiji Seika Kaisha Ltd., Odawara, Kanagawa (Japan). Pharmaceutical Technology Labs.), Watanabe, M, Sumida, N, Okakura, K, Murakami, T
Format Journal Article
LanguageEnglish
Published Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2003
Japan Society for Bioscience Biotechnology and Agrochemistry
Oxford University Press
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Abstract The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.
AbstractList The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.
Author Murakami, T
Okakura, K
Watanabe, M
Sumida, N
Moriya, T. (Meiji Seika Kaisha Ltd., Odawara, Kanagawa (Japan). Pharmaceutical Technology Labs.)
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Cites_doi 10.1007/978-94-011-2930-5_2
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Issue 6
Keywords Enzyme
Gene overexpression
protoplast
Humicola insolens
Humicola
Fungi
avi2
Cellulase
Glycosidases
Hydrolases
Fungi Imperfecti
O-Glycosidases
Thallophyta
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Snippet The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with...
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SubjectTerms avi2
Biological and medical sciences
CELLULASE
Cellulases - biosynthesis
Cellulases - genetics
Cellulases - metabolism
Cloning, Molecular
DNA, Fungal
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genetic Vectors
HUMICOLA
Humicola insolens
Mitosporic Fungi - genetics
MOLECULAR CLONING
NUCLEOTIDE SEQUENCE
protoplast
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transformation, Genetic
TRANSGENICS
Title Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977
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Volume 67
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