Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977

• Published in: Bioscience, biotechnology, and biochemistry Vol. 67; no. 6; pp. 1434 - 1437
• Main Authors: Moriya, T. (Meiji Seika Kaisha Ltd., Odawara, Kanagawa (Japan). Pharmaceutical Technology Labs.), Watanabe, M, Sumida, N, Okakura, K, Murakami, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2003; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: avi2; Biological and medical sciences; CELLULASE; Cellulases - biosynthesis; Cellulases - genetics; Cellulases - metabolism; Cloning, Molecular; DNA, Fungal; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Genetic Vectors; HUMICOLA; Humicola insolens; Mitosporic Fungi - genetics; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; protoplast; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transformation, Genetic; TRANSGENICS; Enzyme; Gene overexpression; protoplast; Humicola insolens; Humicola; Fungi; avi2; Cellulase; Glycosidases; Hydrolases; Fungi Imperfecti; O-Glycosidases; Thallophyta
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.67.1434

The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.