BACs as Tools for the Study of Genomic Imprinting

Genomic imprinting in mammals results in the expression of genes from only one parental allele. Imprinting occurs as a consequence of epigenetic marks set down either in the father's or the mother's germ line and affects a very specific category of mammalian gene. A greater understanding o...

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Published inBioMed research international Vol. 2011; no. 2011; pp. 1 - 10
Main Authors Tunster, S. J., John, R. M., Van De Pette, M.
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Puplishing Corporation 01.01.2011
Hindawi Publishing Corporation
John Wiley & Sons, Inc
Hindawi Limited
Subjects
Advantages
Animals
Biomedical Research
Chromosomes, Artificial, Bacterial
Deoxyribonucleic acid
DNA
DNA methylation
E coli
Genetic aspects
Genetic engineering
Genomic Imprinting
Genomics
Humans
Insulin-like growth factor 1
Mice
Physiological aspects
Review
Studies
United Kingdom
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Summary:Genomic imprinting in mammals results in the expression of genes from only one parental allele. Imprinting occurs as a consequence of epigenetic marks set down either in the father's or the mother's germ line and affects a very specific category of mammalian gene. A greater understanding of this distinctive phenomenon can be gained from studies using large genomic clones, called bacterial artificial chromosomes (BACs). Here, we review the important applications of BACs to imprinting research, covering physical mapping studies and the use of BACs as transgenes in mice to study gene expression patterns, to identify imprinting centres, and to isolate the consequences of altered gene dosage. We also highlight the significant and unique advantages that rapid BAC engineering brings to genomic imprinting research.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
Academic Editor: Noelle E. Cockett
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2011/283013