Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

• Published in: Nanomaterials (Basel, Switzerland) Vol. 10; no. 2; p. 390
• Main Authors: Diniz, F R, Maia, R C A P, Rannier, L, Andrade, L N, V Chaud, M, da Silva, C F, Corrêa, C B, de Albuquerque Junior, R L C, P da Costa, L, Shin, S R, Hassan, S, Sanchez-Lopez, E, Souto, E B, Severino, P
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.02.2020; MDPI
• Subjects: Activity recognition; Alginic acid; Antimicrobial activity; Antimicrobial agents; Bactericidal activity; Biopolymers; Bonding strength; Cell adhesion & migration; Cell division; Collagen; Cytotoxicity; Fourier analysis; Fourier transforms; Gelatin; Granulation; healing; Hydrogels; Hydrogen bonding; Hydrogen bonds; In vivo methods and tests; Infrared analysis; Injury prevention; Laboratory animals; Minimum inhibitory concentration; Nanoparticles; Natural polymers; Polymers; Pseudomonas aeruginosa; Silver; silver nanoparticles; Sodium; Sodium alginate; Therapeutic applications; Tissues; Transmission electron microscopy; Wound healing; Brazil; United States > US; Japan; Germany; sodium alginate; healing; antimicrobial activity; silver nanoparticles; gelatin
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano10020390

Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430-450 nm in the ultraviolet-visible (UV-Vis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FT-IR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm . Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against and 53.0 µg/mL against s. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications.