Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan

• Published in: Antonie van Leeuwenhoek Vol. 107; no. 1; pp. 251 - 261
• Main Authors: Corona-González, R. I, Pelayo-Ortiz, C, Jacques, G, Guatemala, G, Arriola, E, Arias, J. A, Toriz, G
• Format: Journal Article
• Language: English
• Published: Cham Springer-Verlag 01.01.2015; Springer International Publishing; Springer Nature B.V
• Subjects: aeration; Agave; Agave - chemistry; Agave - microbiology; Agave tequilana; Bacteria; beta-fructofuranosidase; beta-Fructofuranosidase - metabolism; Biomedical and Life Sciences; Enzymatic activity; enzyme activity; Experimental design; Flowers & plants; Fructans - metabolism; Glycoside Hydrolases - metabolism; Hydrogen-Ion Concentration; Hydrolysis; Life Sciences; Medical Microbiology; Microbiology; mixing; Original Paper; Plant Sciences; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Soil Science & Conservation; Strain rate; Substrates; Temperature; Tequila; fructan; Fructanase; Enzymes
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0323-0

A new wild strain of Saccharomyces cerevisiae (CF3) isolated from tequila must was evaluated for production of fructanase on Agave tequilana Weber fructan (FT). Fructanase activity (F) was assessed by a 3³factorial design (substrate, temperature and pH). High enzymatic activity (31.1 U/ml) was found at 30 °C, pH 5, using FT (10 g/l) as substrate. The effect of initial substrate concentration on F (FT₀, 5.7–66 g/l) was studied and it was found that F was highest (44.8 U/ml) at FT₀25 g/l. A 2²factorial experimental design with five central points was utilized to study the effect of stirring and aeration on fructanase activity; stirring exhibited a stronger effect on F. The ratio fructanase to invertase (F/S) was 0.57, which confirms that the enzymes are fructanase. Crude fructanase reached high substrate hydrolysis (48 wt%) in 10 h. It is shown that S. cerevisiae CF3 was able to produce large amounts of fructanase by growing it on fructan from A. tequilana.