Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan
A new wild strain of Saccharomyces cerevisiae (CF3) isolated from tequila must was evaluated for production of fructanase on Agave tequilana Weber fructan (FT). Fructanase activity (F) was assessed by a 3³factorial design (substrate, temperature and pH). High enzymatic activity (31.1 U/ml) was found...
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Published in | Antonie van Leeuwenhoek Vol. 107; no. 1; pp. 251 - 261 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer-Verlag
01.01.2015
Springer International Publishing Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A new wild strain of Saccharomyces cerevisiae (CF3) isolated from tequila must was evaluated for production of fructanase on Agave tequilana Weber fructan (FT). Fructanase activity (F) was assessed by a 3³factorial design (substrate, temperature and pH). High enzymatic activity (31.1 U/ml) was found at 30 °C, pH 5, using FT (10 g/l) as substrate. The effect of initial substrate concentration on F (FT₀, 5.7–66 g/l) was studied and it was found that F was highest (44.8 U/ml) at FT₀25 g/l. A 2²factorial experimental design with five central points was utilized to study the effect of stirring and aeration on fructanase activity; stirring exhibited a stronger effect on F. The ratio fructanase to invertase (F/S) was 0.57, which confirms that the enzymes are fructanase. Crude fructanase reached high substrate hydrolysis (48 wt%) in 10 h. It is shown that S. cerevisiae CF3 was able to produce large amounts of fructanase by growing it on fructan from A. tequilana. |
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Bibliography: | http://dx.doi.org/10.1007/s10482-014-0323-0 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-6072 1572-9699 |
DOI: | 10.1007/s10482-014-0323-0 |