Ruminococcaceae_UCG-013 Promotes Obesity Resistance in Mice

• Published in: Biomedicines Vol. 10; no. 12; p. 3272
• Main Authors: Feng, Jinlian, Ma, Hongliang, Huang, Yiting, Li, Jiangchao, Li, Weidong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2022; MDPI
• Subjects: 16S rRNA gene sequencing; Animal models; Antibiotics; Biomarkers; Body weight gain; Carbohydrates; Cholesterol; Correlation analysis; Diabetes mellitus (non-insulin dependent); Diet; Digestive system; Epidemiology; Experiments; Feces; Gastrointestinal tract; Germfree; Glucose; High density lipoprotein; High fat diet; intestinal flora; Intestinal microflora; Laboratory animals; Low density lipoprotein; Metabolism; Microbiomes; Microbiota; obese prone; obese resistant; Obesity; Phenotypes; Phenotypic variations; Physiological aspects; Proteins; rRNA 16S; Ruminococcaceae; Ruminococcaceae_UCG-013; Transplantation; Variance analysis; Weight control; China; United States > US; Ruminococcaceae_UCG-013; 16S rRNA gene sequencing; obese prone; obese resistant; intestinal flora
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines10123272

Alterations in the gut microbiome have been linked to obesity and type 2 diabetes, in epidemiologic studies and studies of fecal transfer effects in germ-free mice. Here, we aimed to identify the effects of specific gut microbes on the phenotype of mice fed a high-fat diet (HFD). After eight weeks of HFD feeding, male mice in the HFD group ranking in the upper and lower quartiles for body weight gain were considered obese prone and obese resistant, respectively. 16S rRNA gene sequencing was used to determine the composition of the intestinal microbiota, and fecal transplantation (FMT) was conducted to determine whether the microbiota plays a causal role in phenotypic variation. Ruminococcaceae_UCG-013 was more abundant in the gut microbes of mice with a lean phenotype than in those with an obese phenotype. Ruminococcaceae_UCG-013 was identified as the most significant biomarker for alleviating obesity by random forest analysis. In a correlation analysis of serum parameters and body weight, Ruminococcaceae_UCG-013 was positively associated with serum HDL-C levels and negatively associated with serum TC, TG, and LDL-C levels. To conclude, Ruminococcaceae_UCG-013 was identified as a novel microbiome biomarker for obesity resistance, which may serve as a basis for understanding the critical gut microbes responsible for obesity resistance. Ruminococcaceae_UCG-013 may serve as a target for microbiome-based diagnoses and treatments in the future.