Characteristics of persistent Salmonella enteritidis strains in two integrated broiler chicken operations of Korea

• Published in: Journal of Veterinary Medical Science Vol. 70; no. 10; pp. 1031 - 1035
• Main Authors: Kwag, S.I.(Ministry of Agriculture and Forestry, Gwacheon (Korea R.). Animal Health Division), Bae, D.H, Cho, J.K, Lee, H.S, Ku, B.G, Kim, B.H, Cho,G.J, Lee, Y.J
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.10.2008; Japan Science and Technology Agency
• Subjects: Animals; Bacterial Proteins - genetics; Bacteriophage Typing - veterinary; BROILER CHICKENS; chicken; Chickens; Cluster Analysis; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field - veterinary; Enterotoxins - genetics; EPIDEMIOLOGIA; EPIDEMIOLOGIE; EPIDEMIOLOGY; FENOTIPOS; Fimbriae Proteins - genetics; GENOTIPOS; GENOTYPE; GENOTYPES; Korea - epidemiology; Microbial Sensitivity Tests - veterinary; PHENOTYPE; PHENOTYPES; POLLO DE ENGORDE; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; POULET DE CHAIR; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; Prevalence; REPUBLIC OF KOREA; REPUBLICA DE COREA; REPUBLIQUE DE COREE; SALMONELLA ENTERITIDIS; Salmonella enteritidis - genetics; Salmonella enteritidis - growth & development; Salmonella enteritidis - isolation & purification; Salmonella enteritidis - pathogenicity; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; Korea
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.70.1031

The objectives of the study were to investigate the phenotypic and genotypic characterization of the persistent Salmonella Enteritidis (S. Enteritidis) isolates in two integrated broiler chicken operations, with attention focused mainly on the epidemiological approach. In the distribution of virulence genes, Salmonella enterotoxin (stn), invading host cell (invA), and Salmonella plasmid virulence (spvC) genes were widely distributed among the S. Enteritidis irrespective of their source of isolation, and Salmonella fimbrial (sefC) and plasmid encoded fimbrial (pef) genes were present in 28 and 20 S. Enteritidis strains, respectively. A total of 5 different XbaI-PFGE types were obtained from 31 S. Enteritidis isolates. Twenty-one types were divided on the basis of their PFGE pattern, phage type and antimicrobial resistance pattern determined. There was a significant difference in phenotypic and genotypic characterization by two integrated broiler operations. Also, 8 isolates susceptible to all antimicrobials and 11 isolates with resistance to nalidixic acid were partly classified by XbaI PFGE pattern and by the phage type.