Subclinical Mastitis and Antimicrobial Susceptibility of Staphylococcus caprae and Staphylococcus epidermidis Isolated from Two Italian Goat Herds

• Published in: Journal of dairy science Vol. 88; no. 5; pp. 1694 - 1704
• Main Authors: Moroni, P, Pisoni, G, Antonini, M, Ruffo, G, Carli, S, Varisco, G, Boettcher, P
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.05.2005; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: Amoxicillin - pharmacology; Animal productions; Animals; Anti-Bacterial Agents - pharmacology; antibiotic resistance; antimicrobial susceptibility; Biological and medical sciences; Cell Count; chronic diseases; Clavulanic Acid - pharmacology; coagulase negative staphylococci; dairy animals; disease prevalence; Female; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; goat; Goat Diseases - epidemiology; Goat Diseases - microbiology; Goats; Mastitis - epidemiology; Mastitis - microbiology; Mastitis - veterinary; Microbial Sensitivity Tests; Milk - cytology; Milk - microbiology; Penicillin G - pharmacology; somatic cell count; Staphylococcal Infections - drug therapy; Staphylococcus - drug effects; Staphylococcus - isolation & purification; Staphylococcus caprae; Staphylococcus epidermidis; Staphylococcus epidermidis - drug effects; Staphylococcus epidermidis - isolation & purification; Terrestrial animal productions; Vertebrates; Italy; antimicrobial susceptibility; SCAP; goat; SEPI; somatic cell count; coagulase-negative staphylococci; Dairy animal; Mastitis; Somatic cell; Herd; Antimicrobial agent; Vertebrata; Mammalia; Sensitivity resistance; Staphylococcus epidermidis; Dairy industry; Bacteria; Micrococcales; Micrococcaceae; Goat; Artiodactyla; Ungulata
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(05)72841-1

A total of 156 goats from 2 commercial dairy goat farms were monitored for intramammary infections during an entire lactation. Most of the infections (80.7%) observed were due to coagulase-negative staphylococci (CNS) species. In herd 1, nearly all of the infections (96%) were due to CNS species, with Staphylococcus caprae (SCAP) being the most common specific pathogen observed, accounting for about 43% of the infections. In herd 2, the proportion of the infections due to CNS was 67% and Staphylococcus epidermidis (SEPI) was the most common pathogen (48% of infections) and SCAP was not present. Linear somatic cell scores (SCS) were greater in milk from infected udder halves, with an average difference of 0.78 SCS. The SCS for infected udder halves was greater than noninfected for all CNS species, although differences among species were observed. The ranking across CNS species was SCAP>other CNS>SEPI>no infection. However, infections by SEPI tended to be more persistent. Increased SCS was associated with a statistically significant decrease in milk yield, but no effect was observed for intramammary infections (IMI). Ninety-seven isolates of CNS (53 SCAP and 44 SEPI) were investigated for in vitro susceptibility to several antimicrobial agents. Benzylpenicillin was the most effective antimicrobial agent against SCAP and SEPI. A concentration of 0.05μg/mL was sufficient to inhibit growth of 90% of SCAP colonies, and 0.10μg/mL yielded a similar effectiveness for SEPI. Amoxicillin and the combination of amoxicillin and clavulanic acid were only slightly less effective. Tetracycline (62.5μg/mL) and tilmicosin (500μg/mL) were the least effective treatments for SEPI and SCAP, respectively.