Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7

• Published in: The Kaohsiung journal of medical sciences Vol. 30; no. 9; pp. 435 - 446
• Main Authors: Saravana Kumar, P, Duraipandiyan, V, Ignacimuthu, S
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.09.2014; Wiley
• Subjects: Actinomycetes; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antimicrobial activity; Chemical Fractionation; Culture Media; DNA, Ribosomal - genetics; Drug Resistance, Bacterial - drug effects; Gas Chromatography-Mass Spectrometry; Internal Medicine; Methanolic extract; Microbial Sensitivity Tests; Minimum inhibitory concentration; Molecular Sequence Data; Phylogeny; Sequence Analysis, DNA; Soil Microbiology; Streptomyces - chemistry; Streptomyces - cytology; Streptomyces - physiology; Streptomyces sp. SCA 7; Methanolic extract; Antimicrobial activity; Actinomycetes; Minimum inhibitory concentration; Streptomyces sp. SCA 7
• ISSN: 1607-551X; 2410-8650
• DOI: 10.1016/j.kjms.2014.05.006

Abstract Thirty-seven actinomycetes strains were isolated from soil samples collected from an agriculture field in Vengodu, Thiruvannamalai District, Tamil Nadu, India (latitude: 12° 54′ 0033″, North; longitude: 79° 78′ 5216″, East; elevation: 228.6/70.0 ft/m). The isolates were assessed for antagonistic activity against five Gram-positive bacteria, seven Gram-negative bacteria, and two pathogenic fungi. During the initial screening, 43% of the strains showed weak activity, 16% showed moderate activity, 5% showed good activity, and 35% showed no antagonistic activity. Among the strains tested, SCA 7 showed strong antimicrobial activity. Maximum biological activity was obtained on modified nutrient glucose agar (MNGA) medium. The mycelia of SCA 7 were extracted with methanol and tested against microbial pathogens using the disc diffusion method. The crude extract was purified partially using column chromatography and assessed for antimicrobial activity. Fraction 10 showed good activity against Staphylococcus epidermidis (31.25 μg/mL) and Malassezia pachydermatis (500 μg/mL) and the active principle (fraction 10) was identified as 2,4-bis (1,1-dimethylethyl) phenol. Based on morphological, physiological, biochemical, cultural, and molecular characteristics (16S rDNA sequencing), this strain was identified as Streptomyces sp. SCA 7. It could be used in the development of new substances for pharmaceutical or agricultural purposes.