Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox

• Published in: Advanced science Vol. 11; no. 38; pp. e2405848 - n/a
• Main Authors: Yang, Huiyi, Zheng, Judun, Wang, Wei, Lin, Jingyan, Wang, Jingru, Liu, Lunjing, Wu, Wenjie, Zhang, Chengli, Zhang, Mingxia, Fu, Yu, Yang, Bin, Liao, Yuhui
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.10.2024; John Wiley and Sons Inc; Wiley
• Subjects: Acids; amplification‐free; Biosensing Techniques - methods; colorimetric‐ECL dual‐mode; Colorimetry - methods; Electrochemical Techniques - methods; Electrons; Humans; Hybridization; Limit of Detection; Luminescent Measurements - methods; Metal-Organic Frameworks - chemistry; monkeypox virus; Morphology; Mpox; Mpox (monkeypox); Onsite; Ru@U6‐Ru/Pt NPs nanotag; signal amplification; Zirconium; Africa; colorimetric‐ECL dual‐mode; Ru@U6‐Ru/Pt NPs nanotag; amplification‐free; signal amplification; monkeypox virus
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.202405848

Dual‐mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive and flexible detection of the monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6‐Ru/Pt NPs is constructed for dual‐mode platforms by integrating double‐layered ECL luminophores and the nanozyme using Zr‐MOF (UiO‐66‐NH2) as the carrier, which not only generates enhanced ECL and colorimetric signals but also provide greater stability than that of commonly used nanotags. Dual‐mode platforms are used within 15 min from the “sample in” to the “result out” steps, without nucleic acid amplification. The colorimetric mode allows the screening of MPXV with the visual limit of detection (vLOD) of 0.1 pM (6 × 108 copies µL−1) and the ECL mode supports quantitative detection of MPXV with an LOD as low as 10 aM (6 copies·µL−1), resulting in a broad sensing range of 60 to 3 × 1011 copies·µL−1 (10 orders of magnitude). Validation is conducted using 50 clinical samples, which is 100% concordant to those of quantitative polymerase chain reaction (qPCR), indicating that Ru@U6‐Ru/Pt NPs‐based dual‐mode sensing platforms showed great promise as rapid, sensitive, and accurate tools for diagnosis of the nucleic acid of MPXV and other infectious pathogens. A new nanotag, Ru@U6‐Ru/Pt NPs is constructed for dual‐mode platforms by integrating double‐layered electrochemiluminescence luminophores and the nanozyme using Zr‐MOF as the carrier. This dual‐mode sensing platform shows great promise as a rapid, sensitive, and accurate tool for point‐of‐care testing and clinical diagnosis of nucleic acids of monkeypox virus and other infectious pathogens.