硅油对淀粉样蛋白和多肽积聚的促进作用

本文研究了硅油对淀粉样蛋白和多肽积聚的影响，采用硫磺素T（ThT）荧光分析法和原子力显微镜表征。结果显示，5种淀粉样蛋白／多肽在含有硅油的体系中积聚更快、更充分，表明一定量的硅油能促进这些淀粉样蛋白／多肽的积聚。此外，研究了涡旋操作对硅油污染的引入。结果表明，涡旋能将离心管内壁的硅油引入溶液中，从而促进GAV9肽的积聚。...

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Bibliographic Details
Published in核技术 Vol. 38; no. 8; pp. 51 - 57
Main Author 李佳 雷豪志 代彬 其其格 张益 胡钧
Format Journal Article
LanguageChinese
Published 中国科学院上海应用物理研究所微观界面物理与探测重点实验室嘉定园区 上海201800%中国科学院生物与化学交叉中心 上海200032 2015
Subjects
涡旋
淀粉样蛋白
硅油
积聚
Aggregation
Vibration
积聚
淀粉样蛋白
涡旋
Silicone oil
硅油
Amyloid protein
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Summary:本文研究了硅油对淀粉样蛋白和多肽积聚的影响，采用硫磺素T（ThT）荧光分析法和原子力显微镜表征。结果显示，5种淀粉样蛋白／多肽在含有硅油的体系中积聚更快、更充分，表明一定量的硅油能促进这些淀粉样蛋白／多肽的积聚。此外，研究了涡旋操作对硅油污染的引入。结果表明，涡旋能将离心管内壁的硅油引入溶液中，从而促进GAV9肽的积聚。
Bibliography:31-1342/TL
LI Jia, LEIHaozhi, DAIBin, QIQige, ZHANGYi, HUJun 1 （Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences Jiading Campus, Shanghai 201800, China） 2（The Interdisciplinary Research Center of Biology and Chemistry, Chinese Academy of Sciences, Shanghai 200032, China）
Silicone oil, Amyloid protein, Vibration, Aggregation
Background： The aggregation of amyloid proteins leads to some serious diseases, such as Alzheimer＇s disease, Parkinson＇s disease; silicone oil is identified as biosafety material, and is widely used in industry and daily life, even implantation in vivo. Purpose： The aim is to study the influence of silicone oil on the aggregation of amyloid proteins and peptides. Methods： We measured the fluorescence of thioflavin T（ThT） in the samples and image the aggregated fibers by atomic force microscope （AFM）. Results： Five kinds of amyloid proteins/peptides aggregated faster and more sufficiently in the samples containing silicone
ISSN:0253-3219
DOI:10.11889/j.0253-3219.2015.hjs.38.080501