Microbial production of L-ascorbic acid from D-sorbitol, L-sorbose, L-gulose, and L-sorbosone by Ketogulonicigenium vulgare DSM 4025

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 3; pp. 659 - 662
• Main Authors: Sugisawa, T. (DSM Nutritional Products Ltd., Basel (Switzerland)), Miyazaki, T, Hoshino, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.03.2005; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ASCORBIC ACID; Ascorbic Acid - biosynthesis; BACTERIA; BIOCHEMICAL PATHWAYS; BIOCONVERSION; Biological and medical sciences; CHEMICAL STRUCTURE; Fundamental and applied biological sciences. Psychology; Gluconobacter oxydans - metabolism; Hexoses - metabolism; Ketogulonicigenium vulgare; L-sorbosone; MICROBIAL PROPERTIES; PRODUCTION; SORBITOL; Sorbitol - metabolism; SORBOSE; Sorbose - analogs & derivatives; Sorbose - metabolism; vitamin C; Sorbitol; Ascorbic acid; Ketogulonicigenium vulgare; L-sorbosone; vitamin C; Sorbose; Production; Vitamin; Microorganism
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.69.659

Ketogulonicigenium vulgare DSM 4025, known as a 2-keto-L-gulonic acid producing strain from L-sorbose via L-sorbosone, surprisingly produced L-ascorbic acid from D-sorbitol, L-sorbose, L-gulose, and L-sorbosone as the substrate under a growing or resting condition. As the best result, K. vulgare DSM 4025 produced 1.37 g per liter of L-AA from 5.00 g per liter of L-sorbosone during 4h incubation time at 30 deg C under the resting cell condition having 5.70 g per liter of wet cells. The precursor of L-AA formation from D-sorbitol and L-sorbose, except for L-gulose, was thought to be the putative furanose form of L-sorbosone. This is the first time it is reported that bacteria can produce vitamin C via L-sorbosone.